نتایج جستجو برای: recombinant protein sag1

تعداد نتایج: 1297381  

Journal: :Journal of cell science 1998
F Seeber J F Dubremetz J C Boothroyd

We have genetically engineered Toxoplasma gondii so that its major surface antigen SAG1 is anchored by a human transmembrane domain (SAG1-TM) instead of its natural GPI anchor (SAG1-GPI) in order to initiate studies to address the function of this protein anchor in parasitic protozoa as well as to get insights into the functional role of SAG1. Our results show that SAG1-TM is correctly folded (...

2013
Érica Araújo Mendes Flavio G. Fonseca Bárbara M. Casério Janaína P. Colina Ricardo Tostes Gazzinelli Braulia C. Caetano

The use of recombinant viral vectors expressing T. gondii antigens is a safe and efficient approach to induce immune response against the parasite and a valuable tool for vaccine development. We have previously protected mice from toxoplasmosis by immunizing the animals with an adenovirus expressing the protein SAG1 (AdSAG1) of T. gondii. We are now looking for ways to improve the vaccination s...

Journal: :international journal of nano dimension 0
s. imani department and research center of biology, ihu, tehran, iran. a.m. zand department and research center of biology, ihu, tehran, iran. m. saadati biotechnology research center, baqiyatallah university of medical sciences, tehran, iran. h. honnari department and research center of biology, ihu, tehran, iran. b. maddah department of chemistry, ihu, tehran, iran.

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

Journal: :Clinical and diagnostic laboratory immunology 2005
Klaus-Ingmar Pfrepper Gisela Enders Marion Gohl Doris Krczal Harald Hlobil Doris Wassenberg Erwin Soutschek

To improve serodiagnostic methods for the diagnosis of acute toxoplasmosis during pregnancy, a new test system has been developed and evaluated based on the use of recombinant antigens. Five recombinant Toxoplasma gondii antigens (ROP1, MAG1, SAG1, GRA7, and GRA8) were cloned in Escherichia coli, purified, and applied directly onto nitrocellulose membranes in a line assay (recomLine Toxoplasma)...

Journal: :Experimental parasitology 2010
Veronica M Cóceres Melina Laguia Becher Maximiliano G De Napoli María M Corvi Marina Clemente Sergio O Angel

Recombinant Toxoplasma gondii small heat shock protein HSP20, surface antigen SAG1 and dense granule GRA7 were analyzed by IgG-ELISA with serum samples of Toxoplasma infected humans grouped as I (IgG+, IgM+), II (IgG+, IgM-) and III (IgG-, IgM-). rHSP20 reacted against 80% and 62.5% of serum samples from groups I and II, respectively. rSAG1 was recognized by 85% of the samples from group I and ...

Journal: :Journal of clinical microbiology 2003
Elisa Beghetto Wilma Buffolano Andrea Spadoni Mariassunta Del Pezzo Manlio Di Cristina Olga Minenkova Eskild Petersen Franco Felici Nicola Gargano

The objective of this work was to develop an antibody-specific immunoglobulin G (IgG) avidity assay to discriminate between acute and latent phases of Toxoplasma gondii infection by using recombinant antigens. One hundred twenty-one serum samples from women who developed IgG antibodies against Toxoplasma during pregnancy were used. The IgG avidities of antibodies directed against epitopes carri...

Journal: :The Journal of infectious diseases 2001
M E Grigg J Ganatra J C Boothroyd T P Margolis

To facilitate genotyping of Toxoplasma gondii in vitreous fluid of patients with severe or atypical ocular toxoplasmosis, polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) assays were developed for SAG3 (p43) and SAG4 (p18), 2 single-copy surface antigen genes. Together with strategies for SAG1, SAG2, and B1, multilocus RFLP analyses were performed on PCR-amplified...

2015
Muqing Cao Jue Ning Carmen I Hernandez-Lara Olivier Belzile Qian Wang Susan K Dutcher Yanjie Liu William J Snell

The role of the primary cilium in key signaling pathways depends on dynamic regulation of ciliary membrane protein composition, yet we know little about the motors or membrane events that regulate ciliary membrane protein trafficking in existing organelles. Recently, we showed that cilium-generated signaling in Chlamydomonas induced rapid, anterograde IFT-independent, cytoplasmic microtubule-de...

Journal: :Journal of clinical microbiology 2004
H Pietkiewicz E Hiszczyńska-Sawicka J Kur E Petersen H V Nielsen M Stankiewicz I Andrzejewska P Myjak

Toxoplasma gondii SAG1, GRA1, and GRA7 recombinant antigens may be regarded as tools for the detection of T. gondii immunoglobulin G antibodies in persons with chronic and acute toxoplasmosis. GRA7 is more correlated with acute toxoplasmosis. A combination of these antigens will increase the sensitivity of enzyme-linked immunosorbent assays.

Journal: :journal of sciences, islamic republic of iran 2011
f. mansouri

a sweet water-soluble protein that reminds stable over wide ranges of temperature and ph, brazzein has various applications. its tastes like cane sugar but have no calories. however, the extraction of brazzein from its natural source is expensive and not applicable. in this study we used recombinant dna technology to provide an alternative option for cheaper mass production of brazzein. a brazz...

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