Previous studies have demonstrated that the renal medulla is more susceptible to bacterial infection than the cortex.' Although more than 100,000 organisms are usually required to produce infection when injected directly into the normal renal cortex, fewer than 10 are necessary in the medulla. Many factors contribute to the remarkable ease with which infection is initiated in the renal medulla....

Bibb, William R. (University of North Carolina, Chapel Hill) and W. R. Straughn. Formation of protoplasts from Streptococcus faecalis by lysozyme. J. Bacteriol. 84:1094-1098. 1962.-Incubation of whole cells of Streptococcus faecalis F24 in the presence of the crystalline egg-white lysozyme and appropriate sucrose concentration resulted in the formation of discrete spherical structures. On dilut...

Protoplast wet densities (1.315 to 1.400 g/ml), determined by buoyant density sedimentation in Metrizamide gradients, were correlated inversely with the protoplast water contents (26.4 to 55.0 g of water/100 g of wet protoplast) of nine diverse types of pure lysozyme-sensitive dormant bacterial spores. The correlation equation provided a precise method for obtaining the protoplast water content...

Protoplast preparations made from Saccharomyces cerevisiae by prolonged treatment with snail digestive juice contained fibrils and chitinous bud-scar residues from the original cell wall.

In tobacco (Nicotiana Tabacum) pith, sweet potato (Ipomoea batatas), and carrot (Daucus carota) storage roots, differences were found between cell wall and protoplast peroxidases in their isoenzyme patterns, activity, and reaction to tissue injury.In the pith of elongating tobacco internodes, 90% of total activity was associated with the walls, 80% of which was due to the ionically and covalent...

Switchgrass (Panicum virgatum L.) is a warm-season perennial grass that has received considerable attention as a potential dedicated biofuel and bioproduct feedstock. Genetic improvement of switchgrass is needed for better cellulosic ethanol production, especially to improve cellulose-to-lignin ratios. Cell suspension cultures offer an in vitro system for mutant selection, mass propagation, gen...

Cota-Robles, Eugene H. (University of California, Riverside). Electron microscopy of plasmolysis in Escherichia coli. J. Bacteriol. 85:499-503. 1963.-Escherichia coli cells plasmolyzed in 0.35 m sucrose reveal plasmolysis at one tip of a cell or in the center of dividing cells in which protoplast partition has been complete. Central plasmolysis reveals that protoplast separation can be complete...

Mycobacillin partially quenched the strong fluorescence when 1-anilino naphthalene 8-sulfonate (ANS) was added to protoplast or plasma membrane but is without any effect on weak fluorescence when added to cell-free extract. There are two classes of ANS binding sites on protoplast or plasma membrane of which one class is sensitive to mycobacillin, being competitively abolished by it. Mycobacilli...

/ 11 Abstract / 12 / 13 One prerequisite for plant functional genomic projects is the development of a high-throughput transformation platform. As the / 14 moss Physcomitrella patens can be transformed via PEG-mediated DNA-uptake into protoplasts, a semi-continuous bioreactor / 15 culture of this plant was optimised regarding protoplast isolation efficiencies. Under standard conditions protopla...

The cell wall is the strong, outermost layer of a plant cell, located external to the plasma membrane. The cell wall often far outlives the protoplast which synthesized it. In cork, for example, the wall serves its particular biological role (physical protection of a tree trunk) for many years after the death of the protoplast. However, the walls of living cells are not inert ‘boxes’ but comple...