Procedures for asymbiotic germination and seedling development under in vitro ex conditions were investigated Bletilla striata. Five different media (½ P6668 ¬Phytamax Orchid Maintenance Media, ½ Media with coconut water, P723 PhytoTechnology Seed Sowing MS media) culture sphagnum moss examined their effectiveness 8 weeks on seed of B. Germination occurred all media, however, the best rate was ...

Terrestrial orchids (Orchidaceae) have become objects of concern to conservationists due to the plants' high sensitivity to changes in its environment (Rasmussen, 1995). Surface sterilization of mature seeds before germination eliminates barriers to germination; hypochlorites are most often used for this (Lindén, 1992). The barriers include an almost waterproof testa, and embryo integuments con...

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...