The complete laboratory data of 24 samples with confirmed coronavirus Disease-2019 were retrieved by nucleic acid testing, and the quantification SARS-CoV-2 World Health Organization (WHO) envelope (E-gene), RNA-Dependent RNA Polymerase (RdRP-gene), Nucleocapsid (N-gene) three primers Roche Cobas z 480. Throat or nasopharyngeal swabs reports demonstrated primer Cross Points (CPs) greater than 2...

background: the wilms tumor 1 (wt1) gene is originally defined as a tumor suppressor gene and a transcription factor that overexpressed in leukemic cells. it is highly expressed in more than 80% of acute myeloid leukemia (aml) patients, both in bone marrow (bm) and in peripheral blood (pb), and it is used as a powerful and independent marker of minimal residual disease (mrd);we have determined ...

background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

Acetyl-coenzyme A carboxylase α (ACC-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. ACC-alpha gene is located on Caprine chromosome 11 and is polymorphic in many goat breeds. In the current study, we aimed to find possible single nucleotide polymorphisms (SNPs) in the exon 1 region of the ACC-alpha gene in Iranian Mahabadi goat breed. Genomic DNA was extracted fro...

Introduction and Aim: Polymerase chain reaction (PCR) of 16S rRNA virulence genes associated with Type Three Secretion System (TTSS) has been used as a rapid method for the identification pathogen Enterobacter cloacae in leukemia patients. Rapid diagnosis this becomes necessary starting proper treatment these In current study, we aimed to isolate from patients study TSSS isolates using molecula...

background and aims: lamivudine is amongst the antiviral for drug chronic hepatitis b treatment. during therapy with lamivudine, variants may emerge with ymdd mutation in the reverse transcriptase (rt) region of polymerase gene. this mutation might have a role in drug resistant for hbv. materials and methods: hbv dna extraction from serum sample of 88 patients, were subjected to nested pcr for ...