A method is described for the culture of mononuclear phagocytes in suspension by incubation on a Teflon film to which the cells do not adhere. The characteristics of peritoneal macrophages, bone marrow mononuclear phagocytes, macrophage cell lines, and fibroblasts cultured in this way are similar to those observed after culture on glass or plastic surfaces. Culture of mononuclear phagocytes in ...

The mechanism of recurrent airway obstruction (RAO) in horses was investigated by measuring the membrane domain structure and oxy-redoxy activity in phagocytes isolated from bronchoalveolar lavage fluid (BAL) and from the blood of healthy and RAO horses by electron paramagnetic resonance (EPR). Differences in the activity of intracellular antioxidant enzymes CAT, GPx, and SOD measured in phagoc...

To review the data on the origins, phenotype, and function of embryonic phagocytes that has accumulated over past decade. Most of the relevant articles were selected based on the PubMed database entries. In additional, the Interactive Fly database (http://sdb.bio. purdue.edu/fly/aimain/1aahome.htm), FlyBase (http://flybase.bio. indiana.edu:82/), and TBase (http://tbase.jax.org/) were used to se...

Drosophila phagocytes participate in development and immune responses through their abilities to perform phagocytosis and/or secrete extra-cellular matrix components, antimicrobial peptides, clotting factors and signalling molecules. However, our knowledge of their functional impact on development and host resistance to infection is limited. To address this, we have used a genetic cell ablation...

For half of a century, it has been known that non-professional phagocytes, such as fibroblasts, endothelial, and epithelial cells, are capable of efferocytosis (engulfment of apoptotic cells). Non-professional phagocytes differ from professional phagocytes in the range and efficiency of engulfment. Much of the recognition and underlying signaling machinery between non-professional and professio...

• Discussing how unwanted cells and comparts in living are removed. PtdSer is maintained to the inner side of membrane. exposed on cell surface. recognized by phagocytes for engulfment. exposure /recognition systems work brain. Phospholipids asymmetrically distributed at plasma Phosphatidylserine (PtdSer) exclusively located leaflet membrane while phosphatidylcholine (PtdCho) glycolipids mainly...