The previous studies of the interaction of purine analogs and human erythrocyte acid phosphatase isozymes were extended to include erythrocyte acid phosphatase from seven other species. Consistent responses, similar to the observations with the several genotypically different human isozymes, were observed. The isozyme from chimpanzee erythrocytes was similar to the human B-type isozyme while th...

The cytosolic aldehyde dehydrogenase (ALDH) isozyme from cyclophosphamide (f PA) resistant 1.1210 cells (L1210/CPA) was purified to apparent homogeneity using ternary enzyme complex-dye ligand chromatography. The purified isozyme migrates as a single band at M, 51,000 in sodium dodecyl sulfate polyacrylamide gel electrophoresis and as a single charge species at isoelectric point = 5.8 in isoele...

Adenylate kinase activity (ATP:AMP-phosphotransferase, EC 2.7.4.3) was studied in subcellular extracts of rat liver tissue. Essentially all of the activity, over 150 units per g of tissue, wet weight (1.7 units per mg of protein), was released into the cytosol when the tissue was homogenized in 0.025 M sucrose. Isotonic (0.25 M sucrose) homogenization and separation of subcellular fractions by ...

Recently, it has been reported that mitochondria possess a novel pathway for nitric oxide (NO) synthesis. This pathway is induced when cells experience hypoxia, is nitrite (NO(2)(-))-dependent, is independent of NO synthases, and is catalyzed by cytochrome c oxidase (Cco). It has been proposed that this mitochondrially produced NO is a component of hypoxic signaling and the induction of nuclear...

To determine whether thymidine kinase (TK) isozyme status adds clinically useful information in adult nonHodgkin’s lymphoma (NHL). we have analyzed peripheral blood plasma and lymphocytes of 44 patients with NHL for either TK1 or TK2 isozyme activity. On the basis of isozyme status. patients could be divided into two groups that did not differ significantly with respect to known determinants fo...

Two isozymes of glutamate dehydrogenase were shown to exist in a thermophilic strain of Chlorella pyrenoidosa. The NADPH:NADH activity ratios of the NADHand NADPHspecific isozymes were 1: 5 and 33: 1, and the molecular weights were estimated to be 179,000 and 269,000, respectively. Only the NADH-specific isozyme was detectable in nitrate-cultured cells; the synthesis of the NADPH-specific isozy...