s OBJECTIVES: The performance of the Phoenix system with regard to antimicrobial susceptibility testing (AST) for oxacillin of staphylococci, vancomycin of enterococci, and detection of extended spectrum beta-lactamases (ESBL) of Escherichia coli and Klebsiella spp. was evaluated. METHODS: A total of 278 staphylococci (117 Staphylococcus aureus, 161 coagulase negative staphylococci [CNS] were t...

background and aims: the aim of present study was to investigate the molecular characteristic of staphylococcus aureus to detect mec a gene and to type sccmec in strain isolated from healthy worker at larestan hospital. material and methods: this study was carried out from the 250 nasal swab healthy worker  at larestan hospital. multiplex polymerase chain reaction for meca gene was performed in...

In this paper we report on an experimental evaluation of phenotypic and molecular methods as means for the detection of oxacillin resistance in members of the Staphylococcus sciuri group. A total of 109 S. sciuri group member isolates (92 S. sciuri isolates, 9 S. lentus isolates, and 8 S. vitulinus isolates) were tested by the disk diffusion method, the agar dilution method, the oxacillin salt-...

BACKGROUND Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. METHODS A total of 220 clinical isolates of S. aureus which we...

The penicillin binding protein 2a (PBP2a) latex agglutination test using a blood culture pellet was compared to the oxacillin screen agar method using isolated colonies. For blood cultures positive for Staphylococcus aureus (n = 70), the direct PBP2a test was 18% sensitive and 100% specific. The PBP2a test shows poor sensitivity when used directly with positive blood cultures.

Twelve isolates of Streptococcus pneumoniae with decreased susceptibility by oxacillin screen were susceptible by the FOX panel (Micro Media Systems, Cleveland, Ohio), a commercial microdilution system designed for fastidious organisms. These organisms were found to be moderately susceptible or resistant by broth macrodilution and agar dilution methods. This discrepancy indicates that the FOX p...

In a recent article, Hussain et al. evaluated a commercial latex agglutination test (LA), the MRSA-Screen (Denka Seiken Co., Niigata, Japan), for rapid detection of penicillinbinding protein 2a (PBP2a) in mecA-positive and mecA-negative coagulase-negative staphylococci (CNS) (2). The sensitivity and specificity were 100 and 99.5% (2), respectively, although the MRSA-Screen was initially develop...

The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulase-negative staphylococci (CONS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX30) and oxacillin (OX1), oxacillin agar screen test w...

A total of 200 subjects were screened for carriage of methicillin-resistant Staphylococcus aureus (MRSA) at different sites using oxacillin blood agar and mannitol salt agar with oxacillin. Overall carriage rate was 8.5%, with the highest rate in inpatients (15.6%) while the lowest was seen in health care workers (1.8%). The commonest site of colonization was the anterior nares. Oxacillin blood...

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...