A study was conducted opportunistically to evaluate the potential of rescuing immature oocytes from the ovaries of the Sumatran rhinoceros postmortem. Recovered oocytes (n = 30) were placed in maturation culture for 36 hr and inseminated with frozen-thawed homologous spermatozoa. After culture, evaluation of nuclear maturation status revealed that a large number of oocytes were degenerated (n =...

The present study aims to determine the potential of Bali cattle ovaries as sources oocytes for in vitro embryo production based on different ovarian reproductive statuses. were grouped into 4 categories: with no corpus luteum and dominant follicles (CL-DF-), those (CL+DF-), without but (CL-DF+), follicle (CL+DF+). collected via slicing technique grades (a, b, c, d). oocyte’s maturation was per...

in order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown - gvbd) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. occurrence of gvbd and 17,20β-dihydroxy-4-pregnen-3 -one (dhp) production were followed during an expe...

OBJECTIVE To describe isolation and in vitro maturation of primary oocytes from the ovarian cortex in the presence of hypothalamic pituitary down-regulation. DESIGN Case report. SETTING Tertiary care university-affiliated hospital. PATIENT(S) An 18-year-old patient was given treatment with the ABVD (Adriamycin, bleomycin, vinblastine, and dacarbazine) protocol for Hodgkin's disease. She u...

To describe the morphological and histological changes during the annual reproductive cycle of the gonads of Cyprinion tenuiradius, a total of 341 specimens (102 female and 235 male specimens) were collected monthly from the Rudbal River (in the Qarah Aghaj sub-basin, the Persian Gulf basin), Firuzabad, Fars Province, southern Iran. The randomly sampling was adequate to collect males and female...

background : ovarian cancer is the leading cause of death among gynecological cancers. changes in the methylation of brca1 and brca2 may be an effective mechanism for breast and ovarian cancer. this study evaluates the protein expression and methylation status of brca2 in iranian patients. methods : we assessed 60 mullerian-type ovarian cancers by methylation-specific pcr assays and immunohisto...

The influence of red-pigment-concentrating hormone (RPCH) on ovarian maturation in the red swamp crayfish Procambarus clarkii was studied using both in vivo and in vitro techniques. In vivo, RPCH stimulated ovarian maturation. However, RPCH did not affect the ovary in vitro when only RPCH, muscle and ovarian explants were used. But when RPCH, thoracic ganglia, which are known to contain gonad-s...

polycystic ovarian syndrome is a hetrogenous condition with a broad clinical and pathologic spectrum that may reflect the effects of diverse etiologic factors. polycystic ovarian syndrome is an abnormality in the maturation of the follicles which can result in abnormal composition of follicular fluid. by analysis and comparison of the biochemical composition of human follicular fluid in normal ...

Among all mammals, fetal growth and organ maturation must be precisely synchronized with gestational length to optimize survival at birth. Lack of pulmonary maturation is the major cause of infant mortality in preterm birth. Whether fetal or maternal genotypes influence the close relationship between the length of gestation and lung function at birth is unknown. Structural and biochemical indic...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...