Recent advances in rapid freezing and fixation by freeze substitution have allowed structural cell biologists to apply these reliable modes of sample preparation to a wide range of specimens and scientific problems. Progress in electron tomography has produced cellular images with resolution approaching 4 nm in 3D, but our ability to localize macromolecules in these well-fixed, well-resolved sa...

A novel, colloidal nanogold-based drug delivery system for phenytoin, a well-known anti-epileptic drug with an enhanced efflux via P-glycoprotein, has been proposed in this study. The vital physical properties that would aid in predicting the biological interaction of this system were profiled using various techniques such as UV-Vis, DLS, and TEM in corroboration with theoretical calculations. ...

Oligonucleotides containing a biotin functionality were successfully labelled with a streptavidin nanogold conjugate and subsequently separated and analysed by high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS).

Progress in nanomedicine depends on the development of nanomaterials and targeted delivery methods. In this work, we describe a method for the preferential targeting of gold nanoparticles to a tumor in a mouse model. The method is based on the use of the pH Low Insertion Peptide (pHLIP), which targets various imaging agents to acidic tumors. We compare tumor targeting by nonfunctionalized nanog...

Pseudomonas aeruginosa (P. aeruginosa) is an important opportunistic pathogen that causes serious infections in humans, including keratitis in contact lens wearers. Therefore, establishing a rapid, specific and sensitive method for the identification of P. aeruginosa is imperative. In the present study, the uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification combined with...

An atomic-level strategy is devised to gain insight into the origin of nanogold catalysis by using atomically monodisperse Au(n)(SR)(m) nanoclusters as well-defined catalysts for styrene oxidation. The Au(n)(SR)(m) nanoclusters are emerging as a new class of gold nanocatalyst to overcome the polydispersity of conventional nanoparticle catalysts. The unique atom-packing structure and electronic ...

Oligonucleotides carrying amino, thiol groups, as well as fluorescein, c-myc peptide sequence and nanogold at internal positions were prepared and used for the assembly of bidimensional DNA arrays.

Polymer nanocomposites containing nanometals became a subject of interest due to their bactericidal properties. Different polysaccharides have been used as matrices for nanosilver and nanogold synthesis. In this study, we present novel, environmentally friendly method the preparation sodium alginate/nanosilver/graphene oxide (GOX) alginate/nanogold/graphene GOX characteristics. The formation ap...

Silver and gold have been used for centuries as antimicrobial agents. The aim of the study was to investigate diametral tensile strength, microhardness, ion release and light transmission of experimental resin composites. Flowable dental composite SDR (Dentsply, United Kingdom) was modified by nanogold, nanosilver and silica addition. The metal ion release, light transmission study, microhardne...

Here, we report an intrinsic property of gold nanoparticles (nanogold): they can interact selectively with heparin-binding glycoproteins and inhibit their activity. Gold nanoparticles specifically bound vascular permeability factor/vascular endothelial growth factor (VPF/VEGF)-165 and basic fibroblast growth factor, two endothelial cell mitogens and mediators of angiogenesis resulting in inhibi...