avoiding cytolysis or membrane disruption. After incuba tion, the mixtures were centrifuged, and 105,000 x g supernatants were subjected to gel filtration. The macromolecular peak fractions thus obtained contained about 2% radio activity of the whole cells or 50% radioactivity of the plasma membranes. They showed several physical and chemical features characteristic of acid mucopolysaccharides....

Gargoylism or Hurler's syndrome (Hunter, 1917; Hurler, 1919) is a rare genetically determined disease (McKusick, 1960) appearing in autosomal recessive and sex-linked forms. Two of the six known sulphated mucopolysaccharides are produced in excess, namely chondroitin sulphate B and heparitin sulphate. They accumulate in most organs of the body, especially in the liver and the spleen (Meyer, Gru...

The implication of adenylyl sulfate 3’.phosphate in the synthesis of the sulfated acid mucopolysaccharides has been well documented (l-6). However, the nature of the sulfation step, although studied extensively, is still obscure. Although the introduction of new techniques in nucleotide chemistry has resulted in the synthesis of many of the possible precursors of the acid mucopolysaccharides (7...

Collagen is one of the most common fibers in the extracellular matrix, where sulfated mucopolysaccharides are also located. In addition, sulfated mucopolysaccharides are present in some globet cells and secretory glands. The objective of this article is to develop a new staining method that detects these two macromolecules simultaneously in the same sample. The method described stains tissues i...

1. In view of the suggested association between collagen and acid mucopolysaccharides in the connective tissues, this study was designed to see whether the lathyrus factor, beta-aminopropionitrile, affected the acid mucopolysaccharides as well as inhibiting the normal polymerization of collagen. The changing pattern of these two components of cartilage from normal and lathyritic chick embryos a...

avoiding cytolysis or membrane disruption. After incuba tion, the mixtures were centrifuged, and 105,000 x g supernatants were subjected to gel filtration. The macromolecular peak fractions thus obtained contained about 2% radio activity of the whole cells or 50% radioactivity of the plasma membranes. They showed several physical and chemical features characteristic of acid mucopolysaccharides....