The occurrence of a live hypha inside a dead yeast during the transformation from yeast to mycelium of Paracoccidioides brasiliensis is described.

Associations between yeasts and insect herbivores are widespread, and these inter-kingdom interactions play a crucial role in yeast and insect ecology and evolution. We report a survey of insect attraction to live yeast from a community ecology perspective. In the summer of 2013 we screened live yeast cultures of Metschnikowia pulcherrima, M. andauensis, M. hawaiiensis, M. lopburiensis, and Cry...

To evaluatethe effects of supplementing diets of growing Japanese quails (n=450; 7 days old) with chromium (Cr) yeast and / or mannan oligosaccharides (MOS) on productive performance, carcass traits and the immune system are reported. Diets were a basal diet (control) or the basal diet supplemented with 600 mg Cr-yeast/kg (Cr 1), 1200 mg Cr-yeast/kg (Cr 2), 1.0 g MOS/kg, Cr 1 + MOS or Cr 2 + MO...

The effects of Saccharomyces cerevisiae supplementation (6.6×10(8) cfu) and anhydrous ammonia treatment (3%) of wheat straw (WS) were investigated on in-situ dry matter (DM) degradability, and on rumen fermentation and growth performance of lambs. Rumen-fistulated Menemen sheep fed a diet with and without live yeast were used to assess the DM degradability characteristics of WS and ammonia-trea...

The development of cloning vectors for green fluorescent protein (GFP) and the simplicity of yeast reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growth, cell division, and morphogenesis using live cell fluorescence microscopy. This article focuse...

Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic compositio...

Phagocytic cells, such as neutrophils and macrophages, perform a critical role in protecting organisms from infection by engulfing and destroying invading microbes . Although some bacteria and fungi have evolved strategies to survive within a phagocyte after uptake, most of these pathogens must eventually kill the host cell if they are to escape and infect other tissues . However, we now demons...

Correlative light and electron microscopy (CLEM) is a useful tool for investigating cellular structures at high-resolution. We have extended this technology and developed a new method in which CLEM is combined with live-cell fluorescence imaging, “Live CLEM”. With this method, living cells are first examined by fluorescence microscopy then fixed in situ, and finally the same cells are subjected...

A feeding trial was conducted for 75 days to study the effects of live brewer’s yeast (Saccharomyces cerevisiae) on growth and body composition in post larvae of the giant freshwater prawn, Macrobrachium rosenbergii (de Man). Five diets were formulated containing optimum protein level of 35.24±0.04. These experimental feeds were supplemented with 0.0% (control diet), 0.1%, 0.2%, 0.5% and 1.0% l...