Comparison of natural plant and callus extracts of Solanum trilobatum L. was studied against two bacteria and fungi, for their antimicrobial activity using cup diffusion method. Various solvents such as chloroform, petroleum ether and ethanol were used. The leaf and stem segments of the plant were culturedon Murashige and S koog basal medium supplemented with various growth regulators. Maximum ...

Callus cultures from stem of O. sanctum were induced on slightly modified Murashige and Skoog's (MS) medium and supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 1-2 ppm) and kinetin (kn, 1 ppm). Different extractives of stem, leaf and stem callus of O. sanctum were tested for anticonvulsant activity against standard drug phenytoin using maximal electroshock (MES) model. Ethanol and chlo...

Epigenetic changes accompanying plant cell dedifferentiation and differentiation are reported in 35S ribosomal DNA (rDNA) of tobacco (Nicotiana tabacum). There was a reduction of CG and CNG methylation in both intergenic and genic regions of the rDNA cistron in fully dedifferentiated callus and root compared to leaf. The rDNA hypomethylation was not random, but targeted to particular rDNA gene ...

Abstract—Stevia rebaudiana Bertoni (natural sweetener) belongs to Asteraceae family and can be used as substitute of artificial sweeteners for diabetic patients. Conventionally, it is cultivated by seeds or stem cutting, but seed viability rate is poor. A protocol for callus induction and multiplication was developed to produce large no. of calli in short period. Surface sterilized nodal, leaf ...

The indiscriminate use of commercial antimicrobial drugs has resulted in multiple drug resistance and adverse effects on the host. The present study was aimed to elucidate the effect of different biotic/abiotic elicitors on the growth as well as to enhance the metabolite production in leaf and epicotyl calli of Nigella sativa. Further, antibacterial activity of these callus extracts was evaluat...

The antimicrobial activity of plant extracts were found in folk medicine as essential oils or isolated compounds. Some of these compounds were isolated or obtained by bio-guided isolation after previously detecting antimicrobial activity on the part of the plant. Petunia is an imporant medicinal and ornamental plant.The in vitro callus induction and antibacterial activity of Petunia leaf and ca...

Studies were conducted on the effect of amino acids and growth regulators on the production of artemisinin, an antimalarial compound, in the callus of Artemisia absinthium. Callus was initiated on solid MS medium supplemented with BAP and NAA from leaf explant. For the production of artemisinin, the callus was proliferated on sterile filter paper bridge in MS medium supplemented with different ...

background & aim: investigation of suspension cell culture and callus production in catharanthus roseus. catharanthus roseus is an herbaceous perennial plant belongs to the family apocynaceae. c. roseus is important as commercial medicinal plant because of its alkaloidal content. whole plant contains about 130 alkaloids those are classified as vinca alkaloids. vincristine and vinblastine; t...

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM...

During post-embryonic development, roots can be initiated by a programmed developmental order or by environmental and wound stimulation (Bellini et al., 2014; Xu and Huang, 2014; Birnbaum, 2016; Ikeuchi et al., 2016; Kareem et al., 2016; Lup et al., 2016; Rellan-Alvarez et al., 2016; Steffens and Rasmussen, 2016). De novo root regeneration (DNRR) is a type of plant regeneration to produce adven...