We have used a novel affinity-based proteomics technology to examine the protein signature of small secreted extracellular vesicles called exosomes. The technology uses a new class of protein binding reagents called SOMAmers® (slow off-rate modified aptamers) and allows the simultaneous precise measurement of over 1000 proteins. Exosomes were highly purified from the Du145 prostate cancer cell ...

Let An ⊆ Sn denote the alternating and the symmetric groups on 1, . . . , n. MacMahaon’s theorem [11], about the equi-distribution of the length and the major indices in Sn, has received far reaching refinements and generalizations, by Foata [5], Carlitz [3, 4], FoataSchützenberger [6], Garsia-Gessel [7] and followers. Our main goal is to find analogous statistics and identities for the alterna...

Natural q analogues of classical statistics on the symmetric groups Sn are introduced; parameters like: the q-length, the q-inversion number, the q-descent number and the q-major index. MacMahon’s theorem about the equi-distribution of the inversion number and the reverse major index is generalized to all positive integers q. It is also shown that the q-inversion number and the q-reverse major ...

Glycosylation studies of plasma proteins can reveal information about the onset and progression of diseases, where in the glycan biosynthetic pathways are disturbed as in rheumatoid arthritis (RA). The present study was focused on analysis of O-linked glycoproteins of plasma in RA patients. Two dimensional gel electrophoresis of jacalin bound plasma of RA patients revealed a number of different...

BACKGROUND Differentiation of malignant from benign liver tumors remains a challenging problem. In recent years, mass spectrometry (MS) technique has emerged as a promising strategy to diagnose a wide range of malignant tumors. The purpose of this study was to establish classification models to distinguish benign and malignant liver tumors and identify the liver cancer-specific peptides by mass...

BACKGROUND High-throughput proteomic methods for disease biomarker discovery in human serum are promising, but concerns exist regarding reproducibility of results and variability introduced by sample handling. This study investigated the influence of different preanalytic handling methods on surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) protein pro...

Dementia with Lewy bodies (DLB) is one of the most common neurodegenerative diseases and shares multiple clinical and neuropathological parallels with Alzheimer's (AD) and Parkinson's disease (PD). A variety of clinical signs are suggestive for the diagnosis, and imaging (βCIT SPECT) contributes substantially to the diagnosis. The study reported here was performed in search for a biomarker in t...