Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucro...

Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucro...

The isolation of 55 S particles from mitochondria has been reported. Evidence has been presented which emphasizes the importance of adequate control of contamination during preparation of mitochondria. A satisfactory mitochondrial purification method was attained through the use of radioactive microsomal fractions and electron microscopy to assess the contamination of mitochondria and ribosomes...

Mitochondria can be degraded by autophagy in a process termed mitophagy. The Parkinson-disease-associated ubiquitin ligase Parkin can trigger mitophagy of depolarized mitochondria. However, it remains to be determined how the autophagy machinery is involved in this specific type of autophagy. It has been speculated that adaptor proteins such as p62 might mediate the interaction between the auto...

Mitophagy is thought to play an important role in mitochondrial quality control. Mitochondrial division is believed to occur first, and autophagosome formation subsequently occurs to enwrap mitochondria as a process of mitophagy. However, there has not been any temporal analysis of mitochondrial division and autophagosome formation in mitophagy. Therefore, the relationships among these processe...

Mitochondria can be degraded by autophagy; this process is termed mitophagy. The Parkinson disease-associated ubiquitin ligase Parkin can trigger mitophagy of depolarized mitochondria. However, how the autophagy machinery is involved in this specific type of autophagy remains to be determined. It has been speculated that adaptor proteins such as p62 may mediate interaction between the autophago...

Mitochondria isolated from disrupted carrot tissue oxidize succinate more rapidly than all other substrates tested, have relatively high qo2 values, relatively low cofactor requirements and show respiratory control with ADP only when bovine serum albumin (1 %) is present in the homogenization medium. A protective effect of albumin during the isolation or incubation of mitochondria from insect (...