ige-mediated cell signaling, induced by cross-linking of high affinity receptor for ige (fcεri) in the presence of antigen (ag), is a well known mechanism described for mast cell activation in allergy and hypersensitivity reactions, which induces a spectrum of cellular responses such as secretion and up-regulation of cell surface fcεri. although for several years  ige  binding  to  fcεri  was  ...

Results We have demonstrated that boiling decrease solubility and IgE reactivity of PBS-extracted rice and wheat proteins, but in SDS-extracts this reactivity was only slightly affected. The sera of patients highly positive on the IgE immunoblot, positive in basophil activation and skin prick test with boiled rice components were used for characterizing the IgE-binding proteins separated by 1-D...

Background Buckwheat (Fagopyrum esculentum) is not only common pseudo-cereal in Japan, Korea, and other East Asian countries, but also a health food and substitute for wheat flour in Western countries. Buckwheat allergy is an immunoglobulin E (IgE)-mediated hypersensitivity manifesting as severe and critical symptoms induced by ingestion or inhalation of even a small amount of the flour or food...

BACKGROUND Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. METHODS IgE-binding proteins in dog saliva and dander extract were analysed by imm...

By screening phage display random peptide libraries with purified immunoglobulin E (IgE) from birch pollen-allergic patients, we previously defined peptides mimicking natural IgE epitopes (mimotopes) of the major birch pollen allergen Bet v 1. The present study aimed to define a monovalent carrier for the IgE mimotopes to induce protective antibodies directed to the IgE epitopes, suitable for m...

the aim of the present study was to characterize allergen-specific immunoglobulin e (ige) among children in fez region. eighty one children were recruited from the hospital university center of fez. all of them had completed a questionnaire before taking sera. the sera were used to measure total ige and specific data to proteins of egg's white (ewp), peanuts (pp) and gliadins (g). in last part,...

Background: The cultivation of saffron is expanding through the southeast of Iran, and allergy to saffron pollen occurs in workers involved in processing this plant. We aimed to clone, sequence and express a major allergen involved in saffron pollen allergy, and to compare the recombinant with the natural allergen. Methods: The N-terminal amino acid sequence of Cro s 1, an allergen from saff...

BACKGROUND Red meat allergy presents a novel form of food allergy with severe delayed allergic reactions where IgE antibodies are directed against the carbohydrate α-Gal epitope. Food preparation and processing can influence the allergenicity of proteins. The aim of this study was to characterize the proteomic profile of different beef preparations and to investigate their α-Gal reactivity and ...

background lactoferrin is a multipurpose protein of the transferrin domestic. it is a spherical glycoprotein with a molecular weight of about 80 kda which is widely found in secretory discharges, such as milk, saliva, tear, and nasal secretions. lactoferrin is also found in secondary granules of polymorphonuclear leukocytes (pmn) and is released by some acinar cells. it also shows antibacterial...