A protein kinase has been purified from the bovine corpus luteum by acid precipitation, ammonium sulfate fractionation and chromatography on DEAE-cellulose and hydroxylapatite columns. The enzyme has a molecular weight of approximately 159,000. DEAE-cellulose chromatography resolved the protein kinase activity into two peaks, designated as KI and KII. Both peaks possessed adenosine 3’,5’-monoph...

A protein kinase has been purified from the bovine corpus luteum by acid precipitation, ammonium sulfate fractionation and chromatography on DEAE-cellulose and hydroxylapatite columns. The enzyme has a molecular weight of approximately 159,000. DEAE-cellulose chromatography resolved the protein kinase activity into two peaks, designated as KI and KII. Both peaks possessed adenosine 3’,5’-monoph...

In previous studies of the reactions of fluoride ion with synthetic hydroxylapatite and powdered enamel and dentin (1,2), it was thought that low concentrations of fluoride ion might be affecting the rate of recrystallization of the apatites. Neuman et al. (3) found that fluoride impregnation had no effect on calcium and phosphate exchange of glycol-ashed bone. The experiments described below w...

Fluoridated mouthrinses are not advised for preschool children who may swallow much of the agent. An alternate method of delivering topical fluoride to the interproximal tooth surfaces in these young patients may be fluoridated dental floss. The purpose of this study was to determine the fluoride levels of demineralized primary enamel both before and after the use of a commercially available de...

The superoxide (O2-) forming NADPH oxidase complex of resting phagocytes can be activated in a cell-free system by certain anionic amphiphiles, such as sodium dodecyl sulfate (SDS). For O2- production to occur, the participation of both membrane-associated and cytosol-derived components is required. The purpose of this investigation was to isolate and characterize the membrane component of NADP...