t-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the fusarium species. fusarium species have been recognized as a great agricultural problem. they occur worldwide on a variety of plant hosts and cereal grains. the aim of this study was to investigate t-2 toxin-induced liver injury using in situ perfused rat liver....

Methods Heart Perfusion Protocol Male Sprague Dawley rats were anesthetized. The heart was excised, cannulated, and perfused with Krebs-Henseleit buffer equilibrated with 95% O2 5% CO2 at 370C. A water-filled latex balloon was inserted into the left ventricle and connected to a pressure transducer to record the left ventricular pressure and heart rate. The rate-pressure product (RPP) was used a...

BACKGROUND We investigated whether intermittent blood and crystalloid cardioplegia differentially affect myocardial apoptosis and apoptosis gene-related proteins. METHODS AND RESULTS Rabbit hearts were perfused with Krebs-Henseleit buffer on a Langendorff apparatus. Control hearts (n=6) were perfused for 120 minutes without cardioplegic ischemia. Hearts were arrested for 60 minutes with warm ...

AIM To find an in vitro system for the measurement of the androgenic effects of different extracts of Hibiscus macranthus (Malvaceae) and Basella alba (Basellaceae). METHODS The production of testosterone from testes slices incubated in two media, either Krebs-Henseleit buffer containing 0.5% Bovine serum albumin (BSA) or Dubecco's Modified Eagle's medium-F12 Ham nutrient mixture (DME/Ham F12...

Electric field stimulation (EFS) relaxes human histamine-precontracted airways in vitro. This relaxation is only partly neurally mediated. Nonneural relaxation has been also shown in blood vessels and is due to the generation of oxygen radicals by EFS. In isolated airways the origin of the nonneural component of the relaxation is not clear. Because exogenous catecholamines are oxidized during E...

The behavior of human omental fat cells in vitro has been examined in order to define conditions under which glucose is converted to glyceride-glycerol and glyceride fatty acids. Synthesis of glyceride fatty acids from glucose reached maximal rates only after several hours of incubation in Krebs-Henseleit bicarbonate buffer, with or without added bovine albumin. Conversion of glucose to glyceri...

Background. Ventricular fibrillation has deleterious metabolic and functional consequences for the heart. This study had two purposes: first, to define the effects of ventricular fibrillation during hypoxia on energy metabolism and accumulation of intracellular Na' and, second, to test whether the occurrence of ventricular fibrillation can be predicted from functional or metabolic parameters. M...

1 1 1 0 20 40 60 Incubation time (min) Incubation time (min) Fig. 1. Metabolism of adenosine (A) and effects of adenosine on (a) total urate synthesis and (b) that de nouo Hepatocytes (3.15 mg dry wt./ml) were suspended in Krebs-Henseleit bicarbonate buffer, containing 5 mM-glucose and 2 m d 14C]formate (0.05 Ci/mol), and incubated at 4OoC in the absence (M, 0) or presence (0,O) of 0.1 mwadenos...

1. The effect of D-aldosterone and dexamethasone on kallikrein release was studied in the isolated rat kidney perfused with modified Krebs-Henseleit buffer. 2. Animals received D-aldosterone or dexamethasone (10 microgram/100 g body weight) daily for 3 days. Their isolated kidneys were then perfused with the same steroids at rates of 2.0-2.5 microgram/min for 30 min. 3. Dexamethasone increased ...