background: hepatitis b virus (hbv) causes acute and chronic liver disease worldwide. hbv has eight genotypes (a to h) which is the reflection of its genome with their characteristic geographical distribution. each genotype could have different pathogenic and therapeutic characteristics. there have been few records on hbv genotyping in general population from our region. this study aimed to det...

Drug resistance gene mutations in Hepatitis B virus (HBV) are the main reason for failure of currently used therapeutic nucleoside analogues. Two methods-Pyrosequencing and Sanger sequencing, are most commonly used for HBV genotyping and identification of its mutations, but their advantages of the two methods are undefined. Herein, the two methods were used to identify the HBV genotypes and dru...

Hepatitis B is one of the most common infectious diseases in the world, and 350 million people have been estimated to be chronic hepatitis B virus carriers world-wide. Hepatitis B virus (HBV) has been classified into 8 genotypes (A-H) based on an intergroup divergence of 8% or more in the complete nucleotide sequence. Different genotypes of the hepatitis B virus may influence the clinical outco...

Background: Torque teno virus (TTV) was the first human Anelloviridae detected in a Japanese patient with an unknown type of hepatitis in 1997. TTV is by far the first known single-stranded circular DNA virus infecting human. In spite of its widespread nature in human population, its pathogenesis is still unclear. In addition, information regarding TTV infection in Iranian population is limited...

Genotyping of human hepatitis B virus (HBV) can be used to direct clinically effective therapeutic drug-selection. Herein we report that a quick genotyping method for human HBV was established by a specially designed giant magnetoresistive (GMR) biochip combined with magnetic nanoclusters (MNCs), PCR and line probe assay. Magnetic nanoclusters of around 180 nm in diameter were prepared and modi...

We tested the frequency of occult hepatitis B infection (OBI) among Egyptian healthcare workers (HCWs). We tested 132 HCWs for hepatitis B virus (HBV) DNA by nested polymerase chain reaction (PCR), and hepatitis C virus antibody (anti-HCV) by ELISA. HCV RNA was measured by nested PCR in anti-HCV-positive HCWs. HBV-DNA-positive HCWs were subjected to HBV genotyping. We included 132 HCWs who were...

A simple and precise genotyping system based on PCR using type-specific primers was developed for the determination of genotypes A through F of hepatitis B virus (HBV). This assay system is considered to be a useful tool for the molecular diagnosis of HBV infection and for large-scale surveys.

Abstract Eritrea is an East African multiethnic country with intermediate endemicity for hepatitis B. Our aim was to establish the most prevalent genotypes of B virus (HBV) among patients liver disease. A total 293 Eritrean disease who were surface antigen (HBsAg) positive enrolled. All sera tested transaminases, HBV DNA viral load, and seromarkers including HBsAg, anti-HBcAb (total), HBeAg, an...

AIM To look for a rapid low-cost technique for the detection of HBV variants. METHODS Two patients who underwent orthotopic liver transplantation (OLT) for HBV infection were treated with lamivudine (100 mg daily) and HBV infection recurred in the grafted livers. The patients were monitored intensively for liver enzymes, hepatitis B surface antigen (HBsAg) and HBV DNA in serum. Liver biopsy w...