BACKGROUND We investigated whether obese pregnant women negative for gestational diabetes (GDM) still experience dysglycemia, as indicated by high glycated hemoglobin (Hb A1c) at delivery, and whether this impacts offspring and long-term maternal outcomes. METHODS Data of 462 mother-child pairs of our prospective Programming of Enhanced Adiposity Risk in Childhood - Early Screening (PEACHES) ...

A reference method that specifically measures hemoglobin (Hb) A1c is an essential part of the reference system for the international standardization of Hb A1c/glycohemoglobin. We have developed a new method for quantification, based on the specific N-terminal residue of the hemoglobin beta-chains. Enzymatic cleavage of the intact hemoglobin molecule with endoproteinase Glu-C has been optimized ...

Hemoglobin A1c (Hb A1c) 2 is a key parameter for understanding glycemic control in diabetes. It is used by both clinicians and patients to trigger adjustments in behavior and treatment. In general, clinicians understand Hb A1c and its limitations well, but evidence suggests that patients have difficulty understanding what Hb A1c means and how it relates to glucose. This situation may be particu...

CASE DESCRIPTION A sample submitted for hemoglobin (Hb) evaluation on a 5-day-old premature male infant with intestinal perforation, intraventricular hemorrhage, anemia, and sepsis contained 61.1% Hb A, 23.7% Hb F, 2.1% Hb A2, and peaks of 8.2% and 5.2% in the P2 and P3 regions of the Bio-Rad Variant II HPLC thalassemia assay (Fig. 1). Isoelectric focusing showed Hb A, Hb F, Hb A2, and a band c...

Accurate and timely measurements of hemoglobin A1c (Hb A1c) 2 are an increasingly important challenge for clinical laboratories. As Hb A1c measurements are being used to answer new clinical questions, each new use of Hb A1c imposes requirements for the analytical performance of the methods used to measure it. Until recently, Hb A1c was used only as a key test for monitoring glycemic control in ...

Since the observation of a “fast-moving” hemoglobin (Hb) in diabetic blood specimens by Rahbar in 1968 [1] and the subsequent structural identification of the glucose-“modified” Hb [2], the measurement of erythrocyte glycoHb (Hb A1c) has served as the monitor for long-term glucose control for patients with diabetes mellitus [3]. Column chromatography was one of the first methodologies used for ...

Patients with diabetes mellitus are at risk for developing severe debilitating complications, including retinopathy, nephropathy, neuropathy, myocardial infarction, and stroke. Randomized trials have documented that lowering hemoglobin A1c (Hb A1c) 4 concentrations significantly reduces the onset and rate of progression of microvascular complications (1, 2 ). Moreover, Hb A1c, which is formed b...

The glycation gap (GG), the difference between measured glycosylated hemoglobin (Hb A1c) and Hb A1c predicted from fructosamine, has been used to quantify nonglycemic differences in formation of Hb A1c between individuals, but the technique is controversial (1 ). An increased GG in patients with diabetes has been associated with nephropathy diagnosed primarily by proteinuria (2– 4 ). The GG can...

Approximately 5.1% of the US population has diabetes mellitus, and hemoglobin (Hb) A1c levels are routinely measured to monitor long-term glycemic control in these patients. Many laboratories use ion exchange chromatography for such measurements, and the presence of hemoglobin variants and hemoglobinopathies often results in abnormal peaks on the chromatogram. The goal of this study was to eval...