Preferential interactions between the imidazolium cations of ionic liquids and guanine residues in DNA groove lead to more effective stacking bases even at high temperatures, thus favoring thermal stability DNA.

Despite sharing many common features, adenine-binding and guanine-binding sites in proteins often show a clear preference for the cognate over the non-cognate ligand. We have analyzed electrostatic potential (ESP) patterns at adenine and guanine-binding sites of a large number of non-redundant proteins where each binding site was first annotated as adenine/guanine-specific or non-specific from ...

The interaction of molecules with inorganic substrates is a crucial issue forapplications in molecular electronics. It influences important factors suchas the immobilization efficiency and charge injection through inter-face. Moreover, mechanical aspects connected to unfolding biologicalmolecules are important.We hereby present recent efforts our group tackle these problems,based on density fun...

The study of radiation effects in DNA is a multidisciplinary endeavour, connecting the physical, chemical and biological sciences. Despite being mostly filtered by ozone layer, sunlight still expected to (photo)ionise sizeable yields, triggering an electron removal process formation potentially reactive cationic species. In this manuscript, photoionisation decay channels important tautomeric de...

The guanine insertion enzyme from Escherichia coli catalyzes exchange of guanine located at the first position of the anticodon of tRNA with radioactive guanine (N. Okada and S. Nishimura, unpublished data). tRNA isolated from various tumors, including slowly growing Morris hepatoma 7794A, incorporated considerable guanine with E. coli guanine insertion enzyme, whereas tRNA isolated from all no...

Steady state kinetics of hypoxanthine guanine phosphoribosyltransferase-catalyzed reactions are studied. The results obtained suggest that IMP, GMP, P-Rib-PP, and pyrophosphate bind to the same enzyme form, while hypoxanthine and guanine bind to a different form. Guanine activates IMP-pyrophosphorolysis. During the reaction guanine and IMP are consumed with formation of GM

Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for guanine ranged from 29 to 69 micro M in GPRTase assays; the Ki for hypoxanthine...

Graphite-epoxy composites (GECs) are alternative construction materials for electro­chemical sensors. For these materials, the electron transfer rate constant of some redox reaction depends additionally on stoichiometric relationship between insulating and conducting phases composite. In this work, influence dif­fe­rent ratios araldite/hardener/graphite electrochemical properties GEC electrodes...