Enhancers are DNA elements that augment transcription in cis, independent of distance and orientation. Evidence such as hormone dependent neoplastic cell growth and the stimulation of viral replication by sequences present in enhancers suggests that enhancers may also directly affect DNA replication. We tested this hypothesis in recombinant plasmids by asking whether sequences that stimulated D...

The mouse vascular smooth muscle -actin (SMA) gene enhancer is activated in fibroblasts by transforming growth factor 1 (TGF 1), a potent mediator of myofibroblast differentiation and wound healing. The SMA enhancer contains tandem sites for the Sp1 transcriptional activator protein and Pur and repressor proteins. We have examined dynamic interplay between these divergent proteins to identify c...

on the basis of preliminary in vitro screening tests, a competent strain of pantoea agglomerans ena1 (p. agg. ena1) recovered from soybean nodule was evaluated for its antagonistic activity against macrophomina phaseolina causal agent of charcoal rot of soybean. the results of various in vitro assays showed that p. agg. ena1 is capable of exerting strong antagonistic effect against m. phaseolin...

Unregulated expression of vascular endothelial growth factor-A (VEGF-A) plays an important role in tumor growth. We have identified a cell type-specific enhancer, HS-1100, that contributes to VEGF-A transcriptional activation in tumorigenic glioblastoma cell lines. This enhancer exhibits increased accessibility to DNase I in glioblastoma cell lines that express high levels of VEGF-A but not in ...

Splicing of the last intron (intron D) of the bovine growth hormone pre-mRNA requires the presence of a downstream exonic splicing enhancer (ESE). This enhancer is contained within a 115-nucleotide FspI-PvuII (FP) fragment located in the middle of the last exon (exon 5). Previous work showed that the splicing factor SF2/ASF binds to this FP region and stimulates splicing of intron D in vitro. H...

The studies of PGE2 (prostaglandin E2) biosynthesis have focused primarily on the role of cyclo-oxygenases. Efforts have shifted towards the specific PGE2 terminal synthases, particularly mPGES-1 (microsomal PGE synthase 1), which has emerged as the crucial inducible synthase with roles in pain, cancer and inflammation. mPGES-1 is induced by pro-inflammatory cytokines with studies focusing on t...

topical drug dosage forms such as ointments and creams can be easily removed through wetting, movement and contact. the new bioadhesive formulations with enhanced local anesthetic effects are needed for topical administration. the adhesive capacity of hydroxypropyl methylcellulose (hpmc) was determined by measuring the maximum detachment force and the adhesion work with an auto peeling tester. ...

the aim of this study was evaluation of phytochemical components, antioxidant activity, and antibacterial effects of basil (ocimum basilicum l.) essential oil (beo) in vitro. the lipid oxidation of the meat and antibacterial effects of beo were also evaluated in beef burger product. in this empirical study, essential oil of the basil was isolated by hydrodistillation. then, beo was analyzed by ...