Granzyme B (graB) plays a pivotal role in cytotoxic lymphocyte granule-mediated apoptosis through cleavage of intracellular proteins in target cells. Proteinase inhibitor-9 (PI-9) is a potent inhibitor of graB and is highly expressed in cytotoxic lymphocytes. Here, we show by immunohistochemistry that PI-9 is also abundantly expressed in human testicular Sertoli cells and placental syncytial tr...

Macrobenthic infaunal sampling campaigns that aim at unpreserved “fresh” animals for later biochemical analysis follow one of two strategies: either samples are sieved and sorted on board and individual specimens are frozen, or grab/corer samples are frozen completely and animals are separated from sediments later in the laboratory. This study demonstrates that deep freezing of complete grab sa...

Interactive image segmentation of images has great practical importance. Grab cut is an approach based on optimization by graph cut is implemented by Rother et. al. Grab cut is faster technique and it only requires a rough initial trimap by user interaction. But Grab Cut fails for the images having same foreground and background color Intensity Here we proposed a variation of Grab cut by applyi...

Granzyme B (GraB) induces apoptosis in the presence of perforin. Perforin polymerizes in the cell membrane to form a nonspecific ion pore, but it is not known where GraB acts to initiate the events that ultimately lead to apoptosis. It has been hypothesized that GraB enters the target cell through a perforin channel and then initiates apoptosis by cleaving and activating members of the ICE/Ced-...

CONTENTS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 808 Why Three-Dimensional Plume Delineation is Necessary . . . . . . . . . . . . . . . . . . . . . . . 812 Measurement of Vertical Hydraulic Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 814 One Time Sampling versus P...

Reliable perception is required in order for robots to operate safely in unpredictable and complex human environments. However, reliability of perceptual inference algorithms has been poorly studied so far. These algorithms capture uncertain knowledge about the world in the form of probabilistic belief distributions. A number of Monte Carlo and deterministic approaches have been developed, but ...

The diffusive gradient in thin films (DGT) technique is an in situ passive sampling method that is designed to accumulate labile metal species in environmental systems. DGTs were applied for the determination of time integrated concentrations of low level dissolved metals in waters in areas of ecological concern. The data revealed heavy metal contamination of waters and tracked the contaminatio...

"Dissolved" concentrations of contaminants in sediment porewater (Cfree ) provide a more relevant exposure metric for risk assessment than do total concentrations. Passive sampling methods (PSMs) for estimating Cfree offer the potential for cost-efficient and accurate in situ characterization of Cfree for inorganic sediment contaminants. In contrast to the PSMs validated and applied for organic...

The value of a radon measurement is meaningless unless one knows two additional pieces of information about the measurement; the total uncertainty and the Minimum Detectable Concentration (MDC). These statistics are particularly important when making measurements with devices that capture radon and then are analyzed later; such as grab scintillation cells and charcoal devices. During the delay ...

Passive sampling has recently been developed as an alternative to grab or average automated sampling, in order to obtain at lower cost, more realistic estimates of the average concentrations of organic contaminants in surface waters. The aim of this study was to develop and validate the in situ application of stir bar sorptive extraction (SBSE) as a passive sampling technique for the monitoring...