Currently, genetic typing of microorganisms is widely used in several major fields of microbiological research. Taxonomy, research aimed at elucidation of evolutionary dynamics or phylogenetic relationships, population genetics of microorganisms, and microbial epidemiology all rely on genetic typing data for discrimination between genotypes. Apart from being an essential component of these fund...

Encapsulated Haemophilus influenzae strains belong to type-specific genetic lineages. Reliable capsule typing requires PCR, but a more efficient method would be useful. We evaluated capsule typing by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Isolates of all capsule types (a-f and nontypeable; n = 258) and isogenic capsule transformants (type...

background: hla b27 is a sub class of human leukocyte antigen (hla) class i molecule. it was known as the first genetic risk factor associating with ankylosing spondylitis (as). allele frequency of hla-b27 alleles is different across populations. the present study is aimed to investigate the prevalence of hla-b27 in patients referred to danesh clinical laboratory during 2014 to 2015. methods an...

the prevalence of methicillin resistant staphylococcus aureus (mrsa) and methicillin sensitivestaphylococcus aureus (mssa) carriage among poultry and poultry farmers in malaysia is largely unknown.in the current investigation, chickens and chicken farmers from 30 chicken farms were screened for mrsaand s. aureus carriage. the genetic characteristics of the isolates were determined through multi...

The prevalence of methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitiveStaphylococcus aureus (MSSA) carriage among poultry and poultry farmers in Malaysia is largely unknown.In the current investigation, chickens and chicken farmers from 30 chicken farms were screened for MRSAand S. aureus carriage. The genetic characteristics of the isolates were determined through multi...

The ability to distinguish between Escherichia coli strains is critical for outbreak investigations. Binary typing, based on the presence or absence of genetic material, provides a high-throughput alternative to gel- and PCR-based typing techniques that generate complex banding patterns and lack uniform interpretation criteria. We developed, validated, and determined the discriminatory power of...

Rates of biodiversity loss remain high, threatening the life support system upon which all human depends. In a case study, novel composite index (BCI) in line with Convention on Biological Diversity is established Tyrol, Austria, based available national forest inventory and typing data. Indicators are referenced by ecological modeling, protected areas, unmanaged forests using machine learning ...

This article covers the rapidly advancing field of preimplantation genetic diagnosis (PGD), the molecular genetic analysis of cells taken from embryos formed through in vitro fertilization (IVF). The article focuses on current practices in patient management, relevant IVF and PGD procedures, molecular methods used in the genetic analysis, and technical difficulties that can affect test results....

It is a very important significance to explore the bacterial typing methods rapidly, accurately and understand the genetic relation between isolates for controlling effectively the disease and preventing the dissemination and diffusion of the pathogen. The changes in the genetic material nucleotide sequences result in variation and evolution of bacteria, the development of molecular biology and...

Human leukocyte antigen (HLA) typing from next generation sequencing (NGS) data has the potential for applications in clinical laboratories and population genetic studies. Here we introduce a novel technique for HLA typing from NGS data based on read-mapping using a comprehensive reference panel containing all known HLA alleles and de novo assembly of the gene-specific short reads. An accurate ...