An in vitro root explant tissue culture technique is described for determining susceptibility of tomato (Lycopersicon esculentum Mill.) breeding lines and cultivars to the root-knot nematode Meloidogyne incognita. Root explants were taken from 2-day-old seedlings cultured for 30 days at 28 C on Gamborg's B-5 medium with or without nematode inoculum. The remaining portion of the root and stem fr...

the influence of explant nodal positions on the in vitro shoot growth and proliferation were studied in the two rose cvs. ‘bianca’ and ‘el torro’. third, fourth and fifth nodal explants were cultured on the modified ms medium supplemented with 1.0 and 5.0 mg/l ba. in both the cultivars, higher rate of proliferation (‘bianca’ 3.75; ‘el torro’ 2.65) were obtained from the explants distal to the a...

Because dopamine influences arginine vasopressin (AVP) release, the present studies were designed to ascertain the dopamine receptor subtype that potentiates angiotensin II-induced AVP secretion in cultured hypothalamo-neurohypophysial explants. Dopamine (a nonselective D1/D2 agonist), apomorphine (a D2 ≫ D1 agonist), and SKF-38393 (a selective D1 agonist) dose dependently increased AVP secreti...

Articular cartilage is critical to the normal function of diarthrodial joints. Despite the importance of the tissue and the prevalence of cartilage degeneration (e.g., osteoarthritis), the technology required to noninvasively describe nonuniform deformations throughout the volume of the tissue has not been available until recently. The objectives of the work reported in this paper were to 1) de...

PURPOSE To describe a simple technique of cultivating human corneal epithelial stem cells using an Epilife culture medium under serum- and feeder-free conditions. METHODS Cadaveric donor limbal corneal epithelial cells were cultured on denuded amniotic membranes using an explant technique that was free of serum and feeder cells in the Epilife medium containing a growth supplement of defined c...

context : cumin ( cuminum cyminum l.) as a member of the apiaceae family is one of the most important medicinal plants in the world. objective : an experiment was conducted for evaluation of callus induction optimization in cumin accessions (shahdad, koohbanan, badrood and afghanistan). materials and methods : factorial experiment based on completely randomized design (crd) with three replicati...

Despite intensive research efforts over the past years, regeneration of injured axons in the central nervous system remains elusive. In the quest for neurostimulatory agents that promote regeneration, well-defined models and analysis methods are required. Tissue explant cultures closely resemble the in vivo situation, making them ideal to study the effect of compounds on the neuro-glial network...

Corresponding Author: Sang Un Park Department of Crop Science, University of Chungnam National, 99 Daehak-Ro, Yuseong-Gu, Daejeon, 305764, Korea Email: [email protected] Abstract: Plant tissue culture has proven to be commercially important for the replication of various plants species, including medicinal and ornamental plants. This technique has been used extensively in research for applicatio...