CRISPR-diagnostic assays have gained significant interest in the last few years. This has grown rapidly during current COVID-19 pandemic, where CRISPR-diagnostics been frontline contenders for rapid testing solutions. surge research prompts following question: what exactly are achievable limits of detection and associated assay times enabled by kinetics enzymes such as Cas12 Cas13? To explore t...

BACKGROUND There is interest in newborn screening and diagnosis of lysosomal storage diseases because of the development of treatment options that improve clinical outcome. Assays of lysosomal enzymes with high analytical range (ratio of assay response from the enzymatic reaction divided by the assay response due to nonenzymatic processes) are desirable because they are predicted to lead to a l...

The anti-cancer drug 2',2'-difluoro-2'-deoxycytidine (dFdC) is internally incorporated into DNA in vitro. To determine the effects of this incorporation on DNA structure and function, the beta-cyanoethyl phosphoramidite of dFdC was synthesized and oligodeoxyribonucleotides containing dFdC were made using automated solid phase DNA synthesis techniques. Extension of the coupling time was required...

A novel polyacrylamide gel-based femtolitre microchamber system for performing single-molecule restriction enzyme assay on freely-suspended DNA molecules and subsequent DNA electrostretching by applying an alternating electric field has been developed. We attempted the integration by firstly initiating restriction enzyme reaction on a fluorescent-stained lambdaDNA molecule, encapsulated in a mi...

Cytochrome P450 monooxygenases (P450s) are enzymes known to metabolize a wide variety of compounds including insecticides. Their overexpression leading to enhanced insecticide detoxification could result in insecticide resistance in insects. The increased mRNA expression of two P450 genes, CYP6P7 and CYP6AA3, has been previously observed in laboratory-selected deltamethrin-resistant Ano...

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato and other solanaceous crops in North and Central America and New Zealand. Previous genotyping studies have demonstrated the presence of three different haplotypes of B. cockerelli in the United States corresponding to three geographical regions: Central, Western, and Northwestern. These studies utilize...

Partially purified neutral protease was entrapped in calcium alginate beads and characterized using casein as a substrate. Temperature and pH maxima of the enzyme showed no changes before and after immobilization and remained stable at 50°C and 7.5 respectively. However substrate concentration for maximum enzyme activity of immobilized enzyme was shifted from 0.45% to 0.8%. Reaction time for im...