Bacillus thuringiensis sporulates in a glucose-glutamate medium without concurrent derepression of the tricarboxylic acid cycle. Glutamate appears to regulate tricarboxylic acid cycle activity as well as to influence spore heat resistance and production of dipicolinic acid.

Calcium salts of analogues of dipicolinic acid were substituted for calcium dipicolinate in tests of germination of spores of Bacillus megaferium ATCC 10778. Only 4Hpyran-2,6-dicarboxylic acid was as active as dipicolinic acid. 3-Methyldipicolinic acid and 4-methyl-4H-pyran-2, B-dicarboxylic acid were active but the germination proceeded less rapidly. In the presence of a threshold concentratio...

Bailey, Glen F. (Western Regional Research Laboratory, Albany, Calif.), Saima Karp, and L. E. Sacks. Ultraviolet-absorption spectra of dry bacterial spores. J. Bacteriol. 89:984-987. 1965.-The possibility of obtaining reasonably satisfactory ultraviolet-absorption spectra of dry spores embedded in KBr has been demonstrated. Such spectra show the three peaks characteristic of calcium dipicolinat...

The muramic lactam content of spores of Bacillus sphaericus mutants defective in meso-diaminopimelic acid synthesis increases almost linearly with an increase of meso-diaminopimelic acid concentration in the medium. Since muramic lactam content is a measure of cortex content, the amount of cortex in spores of the mutants can be easily varied by changing the meso-diaminopimelic acid concentrati...

An immunological precipitation system, with rabbit dipicolinyl antibody, was developed for the purpose of evaluating the role of spore dipicolinic acid as a specific haptenic determinant.