A method has been developed for the preparation of streptococcal nuclease B by batch adsorpton to diethylaminoethyl-cellulose. The enzyme is homogeneous with respect to nuclease activity and is suitable for use as an antigen in measurement of anti-deoxyribonuclease B levels in sera.

A rapid procedure for the separation of phospholipids of lung tissue into acidic and nonacidic fractions by means of diethylaminoethyl cellulose acetate microcolumns is described. The fractions are then resolved into individual phospholipids by thin-layer chromatography and quantified by transmission densitometry.

The antigenic properties of Bacillus subtilis flagella and periodate-treated flagellin were compared by using immobilization-inhibition and diethylaminoethyl cellulose binding assay. The results suggest that there are both unique and cross-reactive antibodies elicited by B. subtilis flagella and flagellin.

The chemical syntheses of monoand dimethyl esters of riboflavin phosphate were accomplished by allowing riboflavin to react with the appropriate monoand dimethylchlorophosphoric acids. Riboflavin monomethyl phosphate was found to be coenzymatically active with nicotinamide adenine dinucleotide phosphate-cytochrome c aporeductase, pyridoxine (or pyridoxamine) phosphate apooxidase, and glycolate ...

Chromatographic separation of native DNA from DNA containing single-stranded regions has been used to determine the relative concentrations of structural intermediates generated during chemically induced DNA repair. Single doses of each of ten compounds were administered to rats. After periods ranging from 90 min to 13 days, hepatic DNA was isolated and analyzed by stepwise elution from benzoyl...

Clostridium botulinum type F toxin of proteolytic Langeland strain was purified. Toxin in whole cultures was precipitated with (NH4)2SO4. Extract of the precipitate was successively chromatographed on diethylaminoethyl-cellulose at pH 6,0, O-(carboxymethyl) cellulose at pH 4.9, and finally diethylaminoethyl-cellulose at pH 8.1. The procedure recovered 14 percent of the toxin assayed in the star...

Recently great advances have been made in the preparation of pure myoglobin by the introduction of ion exchange cellulose column chromatography (1, 2). Myoglobin, however, shows a strong tendency to be oxidized to metmyoglobin during isolation procedures. For this reason all myoglobin preparations reported earlier were obtained in the form of metmyoglobin. Therefore, oxymyoglobin has usually be...