The aim of the study was to compare the effects of two different salting procedures on salt uptake, protein and moisture content, water activity (aw) weight yield, colour and quality of herring fillets. One group of herring fillets was brined with 20% brine solution for one day before dry salting while another group was dry salted only. After 27 days of salting, the fillets were desalted. Sampl...

Using a slight overpressure, a urine sample is loaded onto a monolithic photopolymerized sol-gel column that has been derivatized with hydrophobic carbon chains and then the complex urine matrix is washed with aqueous solution. A buffer containing organic solvent is used to elute the adsorbed peptides by an applied voltage and the sample is then introduced into a mass spectrometer by sheath flo...

Salt removal is a prerequisite for electrospray ionization mass spectrometry (ESI-MS) analysis of biological samples. Rapid desalting and a low volume connection to an electrospray tip are required for time-resolved measurements. We have developed a microfabricated desalting device that meets both requirements, thus providing the foundational technology piece for transient ESI-MS measurements o...

Qatar has experienced rapid economic growth due to the discovery and production of fuel oil and natural gas (NG). The natural renewable water resources (rainfall and groundwater [GW]) are depleted; and are estimated as 71-m/per year per capita in 2005. This is far below the water poverty line of 1,000-m/yca. The GW withdrawal is excessive (compared to replenishment) and is used mainly for irrig...

This unit describes the fractionation and analysis of neutral oligosaccharides by high-performance liquid chromatography (HPLC) on bonded amine columns. Separation is based upon hydrogen bonding between the NH2 groups of the column and the hydroxyl groups of the oligosaccharides. A support protocol describes the reduction and desalting of neutral oligosaccharides with sodium borohydride.