Protein structure depends on weak interactions and covalent bonds, like disulfide bridges, established according to the environmental conditions. Here, we present the validation of two spectroscopic methodologies for the measurement of free and unoxidized thiols, as an attribute of structural integrity, using 5,5'-dithionitrobenzoic acid (DTNB) and DyLight Maleimide (DLM) as derivatizing agents...

dynamics simulations of lysozyme unfolding R. Zhou M. Eleftheriou C.-C Hon R. S. Germain A. K. Royyuru B. J. Berne We have performed molecular dynamics simulations for a total duration of more than 10 ls (with most molecular trajectories being 1 ls in duration) to study the effect of a single mutation on hen lysozyme protein stability and denaturing, using a IBM Blue Gene/Le supercomputer. One ...

Type-I procollagen, 14C-biosynthetically labelled, was reduced under denaturing and non-denaturing conditions. Reoxidation to disulphide-linked trimers occurred with non-denatured chains in the presence of an oxidant system containing oxidized and reduced glutathione. Dimeric intermediates were not detected. This reoxidation was accelerated by homogeneous beef liver protein disulphide-isomerase.

Nucleoproteins (NPs) encapsidate the Phlebovirus genomic (-)RNA. Upon recombinant expression, NPs tend to form heterogeneous oligomers impeding characterization of the encapsidation process through crystallographic studies. To overcome this problem, we set up a standard protocol in which production under both non-denaturing and denaturing/refolding conditions can be investigated and compared. T...

conclusions with regard to improving the understanding of the differences in the biodiversity of the fecal microbiota between rd infants and h infants, the findings of this study can provide a possible basis to reveal the relationship between rd and intestinal microbiota. objectives this study aimed to reveal the changes in the biodiversity of the fecal microbiota of infants with rd. patients a...

Different mechanisms have been proposed for the formation of gaseous protein ions during electrospray ionization (ESI). In charged residue model (CRM) are produced upon nanodroplet evaporation to dryness. This mechanism is thought dominate in native ESI, where proteins retain compact conformations, with charge states close Rayleigh protein-sized aqueous droplets. Much higher generated from that...