Isolating and culturing myoblasts is essential for techniques such as tissue regeneration in vitro meat production. This research describes a protocol to isolate primary from skeletal muscle of an adult horse. The equine expressed markers specific had multipotent potential capabilities with differentiation into chondrocytes, adipocytes osteoblasts vitro. horse did not adhere Cytodex 3 grew poor...

In this paper we describe a three-dimensional, multicellular tissue-equivalent model, produced in NASA-designed, rotating wall bioryactors using mammalian cells engineered for genomic containment of mUltiple copies of defined target genes for genotoxic assessment. The Rat 2A fibroblasts (Stratagene, Inc.) were genetically engineered to contain high-density target genes for mutagenesis. Stable t...

Two strains of reovirus (serotype 1 Lang/TIL and serotype 3 Dearing/T3D) were propagated in Vero cells grown in stationary or agitated cultures in a serum-free medium, M-VSFM. Solid microcarriers (Cytodex-1) were used to support cell growth in agitated cultures with a normal doubling time of 25 h. Cell yields of 1 x 10(6) cells/mL were obtained from an inoculum of 2 x 10(5) cells/mL in 4 days i...

BACKGROUND Cytoadherence of Plasmodium falciparum- infected red blood cells to endothelial cells is an important mechanism for parasite survival and a major trigger for diseases pathology. Here, we describe a new adhesion assay in which different cell types (CHO, CHO/CD36 and CHO/ICAM-1) are attached to Cytodex beads in a mini-column format to measure the relative sizes of various binding subpo...

The Vero cell is the continuous line used as a substrate for many viral vaccines manufacturing including Rabies vaccine. Pitman and PV-11 strains of rabies virus are commonly production vaccines. In our study PV - 11 strain seed derived inactivated Anti Vaccines (Lyophilized). working bank lot were prepared tested its sterility mycoplasma contamination. this roller bottle method micro carrier (...

Objective: to study the effectiveness of correcting morphofunctional characteristics liver in an experimental model chronic disease (CLD), using implanted cell-engineered constructs (CECs). Materials and methods. Experiments were carried out on male Wistar rats (n = 80) aged 6–8 months with initial weight 230–250 g. CLD was modeled by inoculating 60% CCl4 oil solution for 42 days based a modifi...

Lipid-derived free radicals were detected by electron paramagnetic resonance (EPR) spectrometry when cultured endothelial cells attached to Cytodex beads were exposed to iron-induced oxidant stress in the presence of the spin trap alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN). Radical adduct formation was enhanced greatly when the cells were supplemented during growth with polyunsaturate...

Vaccinia virus (VACV) is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits...

China is a high population country with millions of animal bite cases every year; thus, it is necessary to explore and develop more effective and productive rabies vaccines for human use. To establish a safe, effective, inexpensive and high-yield rabies vaccine, a non-adjuvant purified Vero cell rabies vaccine produced in the SPEEDA PVRV microcarrier bioreactor was developed by Liaoning Chengda...