beta-Carboline alkaloids are natural products widely distributed in plants and also found in alcoholic beverages, well-cooked foods and tobacco smoke. Various authors have reported genotoxic activities of several carboline in prokaryotic and eukaryotic cells that have been attributed to their abilities to intercalate into DNA. But studies on the genotoxic and on the cytotoxic potencies in human...

The links between diet and genomic instability have been under investigation for several decades, and evidence suggests a significant causal or preventive role for various dietary factors. This study investigates the influence of macronutrients (calories, protein, and glucides) and micronutrients, such as vitamins and minerals, as assessed by a food frequency questionnaire, on genotoxicity biom...

abstract background and aim: increased rate of micronucleus in buccal mucosa cells and its correlation with carcinogenesis is worth consideration. recently, occupation in dental laboratories has been proposed as a predisposing factor. therefore, the present study aimed to evaluate the effect of occupational exposure on buccal mucosa cells of dental laboratory technicians. materials and methods:...

background: benzene is one of the important occupational pollutants. there are some reports about the leukemogenic effects related to low-level exposure to benzene. objective: to study the frequency of micronucleus (mn), nucleoplasmic bridge (nb), and nuclear budding (nbud) in the peripheral blood lymphocytes of petrochemical workers with low level exposure to benzene. methods: we enrolled 50 w...

Cytochalasine B is an indicator in the CBMN assay, but can also be geno- or cytotoxic. The aim of study was to determine optimal dose cytochalasine for Cytokinesis-Block Micronucleus (CBMN) assay domestic cat. results revealed a surprisingly high number micronuclei (MN) relation doses used. It found that concentration 2.5 μg/ml enough obtain binuclear cells, with minimal toxic effect on lymphoc...

Anthracycline antibiotics are widely used in cancer chemotherapy. Doxorubicin and Idarubicin, topoisomerase-targeting anthracyclines, were examined for their effect on chromosomal aberration and micronucleus induction in cultured human lymphocytes employing lymphocyte transformation test and cytokinesis-blocked micronucleus (CBMN) assay. A statistically significant dose-dependent increase in mi...

A mathematical model is described that predicts the effect of altered nuclear/cell division kinetics and cytotoxicity on micronucleus expression in vitro when the micronucleus assay is performed without discriminating between cells that have divided once and cells that have not divided after genotoxic insult. The model is based on the probabilities of: (i) a viable cell completing nuclear divis...

Okadaic Acid (OA) the major diarrheic shellfish poisoning (DSP) toxin is known as a tumor promoter and seems likely implicated in the genesis of digestive cancer. Little is known regarding genotoxicity and carcinogenicity of Domoic Acid (DA), the major Amnesic Shellfish Poisoning (ASP) toxin. Both OA and DA occur in seafood and are of human health concerns. Micronuclei (MN) arise from abnormali...

Background: Stress may cause damages to DNA or/and change the ability of the cells to overcome these damages. It may also cause irregularities in the cell cycle and induce abnormal cell divisions through glucocorticoid-dependent functions. The abnormal cell divisions, in turn, lead to chromosomal mal-segregation and aneuploidy. In this study, the effects of the stress hormone, hydrocortisone (H...