In the present work, we tried to identify mechanism why by which steroid alcohols accumulated when hydroxypropyl-β-cyclodextrin (HP-β-CD) was enhance sterol conversion rate. Compared with bioconversion system without HP-β-CD, reaction rate greatly improved in presence of but largely concurrently. a an enhanced rate, higher intracellular NADH/NAD+ level detected, and production increased also. M...

OBJECTIVES To compare the ability of different cyclodextrin polysulphate (CDPS) derivatives to affect human articular cartilage cell metabolism in vitro. METHODS OA chondrocytes were cultured in alginate and exposed to 5 µg/ml of 2,3,6-tri-O-methyl-β-cyclodextrin (ME-CD), 2,3-di-O-methyl-6-sulphate-β-cyclodextrin (ME-CD-6-S), 2,6-di-O-methyl-3-sulphate-β-cyclodextrin (ME-CD-3-S), (2-carboxyet...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened for the production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneity on a single step by affinity chromatography using insoluble corn starch. The molecular weight of Amy RM44 was estimated to be 66 kDa by SDS-PAGE and zymographic analysis. Nine fold purification was achieved with the specifi...

γ-Cyclodextrin and an enzyme used in its production do not have a history of food use in the EU which means the novel ingredient falls within the scope of novel food Regulation EC No. 258/97. The application for authorisation of γ-Cyclodextrin as a novel food ingredient was prepared pursuant to Commission Recommendation 97/618/EC concerning the scientific aspects and the presentation of informa...

The production of hesperetin glycosides was investigated using glycosylation with Xanthomonas campestris and cyclodextrin glucanotransferase (CGTase). X. campestris glucosylated hesperetin to its 3'-, 5-, and 7-O-glucosides, and CGTase converted hesperetin glucosides into the corresponding maltosides. The resulting 7-O-glucoside and 7-O-maltoside of hesperetin showed inhibitory effects on IgE a...

In this paper, synthesized (raw) multi-walled carbon nanotube (rMWCNTs) surfaces were modified with chitosan and β-cyclodextrin using Chen’s soft cutting technique. Raw and surface modified multi-walled carbon nanotubes were observed by transmission electron microscope (TEM). The results showed that chitosan and β-cyclodextrin could attach to the outer surface of nanotubes, wrapping the nanotub...

A new reliable, fast, and simple method for the detection of aflatoxigenic Aspergillus strains, consisting of the addition of a cyclodextrin (a methylated beta-cyclodextrin derivative) to common media used for testing mycotoxin production ability, was developed. We propose the use of this compound as an additive for fungal culture media to enhance the natural fluorescence of aflatoxins. The pro...