Timolol is a non-selective beta-adrenergic antagonist that is similar to propranolol. The mechanism through which these drugs act on the regression of neovascularization is largely unknown. However, it is thought that the drugs may act through vascular endothelial growth factor signaling, vasoconstriction, and vascular endothelial cell apoptosis. The aim of this study was to determine the effec...

PURPOSE Neovascularization of the avascular cornea is a significant problem associated with many corneal diseases. Because Fas ligand (FasL) is highly expressed in the cornea, the role of this molecule in controlling corneal neovascularization was examined in this study. METHODS C57BL/6(B6), FasL (CD95L)-deficient B6-gld, and Fas (CD95)-deficient B6-lpr mice were subjected to the suture model...

Milani J.F., Barros P.S.M., Guerra J.L. & Brooks D.E. 2008. Effects of topical 0.2% Cyclosporine A on corneal neovascularization induced by xenologous amniotic membrane implantation into a corneal stroma micropocket of rats. Pesquisa Veterinária Brasileira 28(8):379-386. Laboratório de Investigação em Oftalmologia Comparada, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Pau...

Corneal neovascularization (NV) not only reduces visual acuity, but it also causes loss of the cornea's immune privilege, strongly contributing to a worse prognosis in penetrating keratoplasty. Several mediators participate in corneal angiogenesis, and the role of vascular endothelial growth factor (VEGF) has been extensively proven. Anti-VEGF agents have been shown to be effective in slowing t...

PURPOSE To identify differentially expressed genes in corneal neovascularization using cDNA macroarray. METHODS Mechanical denudation of corneal and limbal epithelium was performed to induce corneal vascularization in mice. Corneas were harvested 4 days after operation. Total RNA was isolated from both normal and vascularized corneas and used for the synthesis of cDNA probes. 32P labeled expo...

PURPOSE To determine the efficacy of topical application and subconjunctival injection of bevacizumab in the treatment of corneal neovascularization. METHODS Corneal neovascularization was induced with a silk suture of the corneal stroma in 12 rabbits (24 eyes). One week after suturing, four rabbits were treated with topical bevacizumab at 5 mg/mL (group A) and another four rabbits were treat...

PURPOSE To determine whether subunits of VEGF receptor-1 coupled with an endoplasmic reticulum retention signal can block hypoxia-induced upregulation of VEGF secretion in corneal epithelial cells and block murine corneal angiogenesis induced by corneal injury. METHODS Human corneal epithelial cells, transfected with plasmids encoding Flt23K or Flt24K (the VEGF-binding domains of the Flt-1 re...

OBJECTIVE This study was undertaken to investigate the effects of recombinant human epidermal growth factor (rhEGF) and protein-free calf blood extract on corneal wound healing and neovascularization. MATERIALS AND METHODS An rabbit central corneal wound and neovascularization model was established in rabbits. One eye of each group was chosen randomly for topical administration of protein-fre...

PURPOSE This study was designed to investigate the effects of the locally supplied endogenous angiogenesis inhibitor vasostatin (VS) on corneal angiogenesis. METHODS Recombinant VS was expressed and purified. The effects of VS on the proliferation of endothelial cells were investigated using the methyl thiazolyl tetrazolium (MTT) assay in the absence or presence of angiogenic factors such as ...

purpose: to image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective dna stain, in rats methods: corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 long-evans rats. one week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...