Human serum aryl acylamidase associated with serum cholinesterase was purified to homogeneity. Evidence for the identity of the two enzymes was based on co-elution profiles, co-purification in the different steps including affinity chromatography with constant ratios of specific activity and percentage recoveries, co-migration on gel electrophoresis, parallel inhibition by typical cholinesteras...

A radiometric method has been used on preparations of intact ocular tissue to dissociate the effects of surface cholinesterase from intracellular enzymes. This method provides a technique to determine the effects of surface enzyme potentiation or inhibition, and may be helpful in evaluating the degree of surface enzyme inhibition with pharmacologic response. Surface cholinesterase activity was ...

ABSTRACT The present study was carried out to investigate the effect of benazepril on corneal permeation in goat cornea and its effect on experimentally induced acute and chronic glaucoma in rabbits. Acute glaucoma was produced by i.v. infusion of 5% glucose (15ml / Kg) in rabbits, whereas chronic glaucoma was induced by injection of alpha-chymotrypsin into posterior chamber of rabbit eye. We s...

In 1986, Whittaker wrote ‘Churchill-Davidson’s editorial entitled “The succinylcholine story” in 1963, highlighted probably for the first time in the UK, the role of serum protein polymorphism in drug sensitivity’ [1]. Following a series of detailed family studies, in 1959 Kalow [2] concluded that individuals who had prolonged apnoea following succinylcholine administration were homozygous for ...

Garcinia atroviridis (Clusiaceae) has been used as a postpartum medication agent in folkloric medicine. However, its biological potential has not been fully evaluated. The present study was aimed to evaluate the antioxidant efficacies, cholinesterase enzyme inhibitory and antimicrobial activity of the stem bark extracts of G. atroviridis. Successive extraction was done using solvents of varied ...

Cholinesterase inhibitors are the 'first-line' agents in the treatment of Alzheimer's disease. This article presents the latest information on their pharmacokinetic properties and pharmacodynamic activity. Tacrine was the first cholinesterase inhibitor approved by regulatory agencies, followed by donepezil, rivastigmine and recently galantamine. With the exception of low doses of tacrine, the c...

Cholinesterase activity was estimated in the serum of a Trinidadian female of Asian Indian descent. Her serum cholinesterase was pathologically low (0.17 U/ml) compared to the average reported for the population (10.59 ± 2.77 U/ml), and her dibucaine number could not be estimated. In a pedigree study her sister displayed similar enzyme activity and cholinesterase was 0.09 U/ml. Cholinesterase c...

In this study, a commercially available kit in which propionylthiocholine is used as a substrate and a dibucaine solution to determine the variants of the enzyme were utilized. Plasma cholinesterase activity of 151 patients who underwent different operations was measured during a period of six months. The mean±SD of the enzyme activity in 150 patients was 4.39±1.17 kU/L .. Seventeen patie...

Pesticides can increase productivity in agriculture. Excessive and uncontrolled use of pesticides pose a risk poisoning farmers. Decreased levels cholinesterase enzymes characterize poisoning. The duration spraying is an indicator that affect the enzyme. This study aimed to determine how long affects enzyme farmers who organophosphate pesticides. Examination using enzymatic test method. most co...