Cryptosporidium is an important cause of diarrhea in developed and developing countries, and its epidemiology is of interest. The methodologies used in the detection of Cryptosporidium-specific antibodies vary widely, which complicates comparison of results. This study assesses the performance of a Cryptosporidium recombinant protein (rCP41) in a serological assay compared to that of a crude an...

We produced a recombinant cysteine proteinase of Clonorchis sinensis and tested its value as an antigen for serologic diagnosis of C. sinensis infections. The predicted amino acid sequence of the cysteine proteinase of C. sinensis was 58, 48, and 40% identical to those of cathepsin L cysteine proteinases from Paragonimus westermani, Schistosoma japonicum, and Fasciola hepatica, respectively. We...

Following an outbreak of fascioliasis in Anzali Harbor in 1988, 452 blood samples were collected using a multistage sampling method for hematological and serological studies. After determination of eosinophilia in the samples, antifasciolia antibodies in the sera were also determined using enzyme linked immuno sorbant assay (ELISA) and counter-current immuno electrophoresis (CCIE). 64.2% ...

BACKGROUND Schistosomiasis mansoni is a debilitating and sometimes fatal disease. Accurate diagnosis plays a key role in patient management and infection control. However, currently available parasitological methods are laborious and lack sensitivity. The selection of target antigen candidates has turned out to be a promising tool for the development of more sensitive diagnostic methods. In our...

This report was written by Dr. Donald Fraser of the Department of Paediatrics, Universit!/ of Toronto and The Hospital for Sick Children, Toronto, Ontario, Canada, in consultation with tile Conirnittee Ofl Nutrition of the American Academy of Pediatrics. The /)OSltiOhb taken on the issue examined reflects the current opinion of tile Committee and i)rovi(les tile .ccie iti/ic background for the ...

This report was written by Dr. Donald Fraser of the Department of Paediatrics, Universit!/ of Toronto and The Hospital for Sick Children, Toronto, Ontario, Canada, in consultation with tile Conirnittee Ofl Nutrition of the American Academy of Pediatrics. The /)OSltiOhb taken on the issue examined reflects the current opinion of tile Committee and i)rovi(les tile .ccie iti/ic background for the ...

An antigen common to members of the family Enterobacteriaceae detectable only by means of the hemagglutination test was recently described (1-3). I t was discovered during the course of a study using this test concerned with serologic interrelationships among the almost 145 known Escherickia coli 0 antigen groups (2). Good correspondence was obtained between O antigen groups and homologous rabb...

background: hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of echinococcus granulosus. diagnosis of hydatidosis is still an unresolved problem. serological tests using crude antigens for diagnosis of e. granulosus are sensi-tive, however their specificity are not satisfactory. therefore, who recommended spe-cific serological methods using specific antigens,...

following an outbreak of fascioliasis in anzali harbor in 1988, 452 blood samples were collected using a multistage sampling method for hematological and serological studies. after determination of eosinophilia in the samples, antifasciolia antibodies in the sera were also determined using enzyme linked immuno sorbant assay (elisa) and counter-current immuno electrophoresis (ccie). 64.2% of the...

Bovine herpesvirus (infectious bovine rhinotracheitis virus)-infected cell antigens were solubilized with Nonidet P-40. The crude antigen extract was separated by reaction with bovine hyperimmune serum in line immunoelectrophoresis; individual immunoprecipitates were used to immunize rabbits. Rabbit sera possessing serum neutralizing activity were analyzed by reaction with crude antigen extract...