Purified BLRV RNA was converted to cDNA and cloned into pT7T3 19U phagemid. The nucleotide sequence of a putative bean leafroll luteovirus coat protein gene was determined from the clones pBL9, pBL29 and pBL32 by the chain termination method (1). Sequence analysis reveals a 588 bp open reading frame and an internal 426 bp ORF (Fig. 1). The large ORF is equivalent to a protein of Mr 21.98 kDa, t...

The putative RNA-dependent RNA polymerase of potato leafroll luteovirus (PLRV) is expressed by -1 ribosomal frameshifting in the region where the open reading frames (ORF) of proteins 2a and 2b overlap. The signal responsible for efficient frameshift is composed of the slippery site UUUAAAU followed by a sequence that has the potential to adopt two alternative folding patterns, either a structu...

The helical junction region of a -1 frameshift stimulating hairpin-type mRNA pseudoknot from sugarcane yellow leaf virus (ScYLV) is characterized by a novel C27.(G7-C14) loop 2-stem 1 minor groove base triple, which is stacked on a C8+.(G12-C28) loop 1-stem 2 major groove base triple. Substitution of C27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site...

legume cultivation and production including chickpea is one of the main agricultural objectives in iran. chickpea crop is a good sources of protein consumption for human diet and play a significant role in nitrogen fixation in cultivated soil and also self reliance for iran economy. chickpea cultivated as a non irrigated crop in lorestan and hamadan provinces in iran. several virus diseases inf...

R of the genetic code can be temporarily suspended to make room for a variety of specific tricks that direct synthesis of additional proteins from mRNAs that would not be predicted by their sequences. This ‘‘recoding’’ includes specification of the 21st encoded amino acid, selenocysteine (1, 2). In another form, recoding itself provides a sensing mechanism to regulate expression (3). In other c...