OBJECTIVE Autophagy is a process for turnover of intracellular organelles and molecules that protects cells during stress responses. We undertook this study to evaluate the potential roles of Unc-51-like kinase 1 (ULK1), an inducer of autophagy, Beclin1, a regulator of autophagy, and microtubule-associated protein 1 light chain 3 (LC3), which executes autophagy, in the development of osteoarthr...

OBJECTIVE The aim of the present study is to investigate the differential expression of Beclin1, HMGB1, p62, survivin, ERCC1 and BRCA1 protein in epithelial ovarian cancer (EOC) and to evaluate the relationship between autophagy and platinum resistance of EOC patients during platinum-based chemotherapy with the protein expression. PATIENTS AND METHODS Expression of Beclin1, HMGB1, p62, surviv...

www.landesbioscience.com Cell Cycle 3453 Members of the Bcl-2 protein family that contain only the Bcl-2 homology (BH) domain 3 are inducers of cell death when overexpressed. One of these BH3only proteins, Bmf, was discovered because of its binding to the anti-apoptotic member Bcl-2, BclXL , Bcl-w, and Mcl-1. It is expressed in the pancreas, liver, kidney, and hematopoietic tissues and in many ...

BACKGROUND Beclin1 is an important, primary molecule for autophagy. OBJECTIVES It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV. MATERIALS AND METHODS In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluat...

BACKGROUND Aberrant α-synuclein aggregation due to the deficiency of ubiquitin-proteasome or of autophagy characterizes the parkinson disease (PD). High mobility group box 1 (HMGB1) is a novel stress sensor to mediate the persistent neuro-inflammation and the consequent progressive neurodegeneration, via controlling the cellular autophagy/apoptosis checkpoint during inflammation. Moreover, HMGB...

BACKGROUND FK506 binding protein 51 (FKBP51) is an Hsp90 co-chaperone and regulator of the glucocorticoid receptor, and consequently of stress physiology. Clinical studies suggest a genetic link between FKBP51 and antidepressant response in mood disorders; however, the underlying mechanisms remain elusive. The objective of this study was to elucidate the role of FKBP51 in the actions of antidep...

Patients with ovarian cancer (OC) may be treated with surgery, chemotherapy and/or radiation therapy, although none of these strategies are very effective. Several plant-based natural products/dietary supplements, including extracts from Emblicaofficinalis (Amla), have demonstrated potent anti-neoplastic properties. In this study we determined that Amla extract (AE) has anti-proliferative effec...

Recent research has revealed a role for Ambra1, an autophagy-related gene-related (ATG) protein, in the autophagic pro-survival response, and Ambra1 has been shown to regulate Beclin1 and Beclin1-dependent autophagy in embryonic stem cells. However, whether Ambra1 plays an important role in the autophagy pathway in colorectal cancer cells is unknown. In this study, we hypothesized that Ambra1 i...

Osteosarcoma (OS) is the most common cancer of the bone. Chemotherapy is commonly used for the clinical treatment of OS. However, chemoresistance to cisplatin [also known as diamminedichloridoplatinum (II) (DDP)] is a major obstacle for OS therapy, the underlying mechanism of which is not fully understood. The present study aimed to investigate the role of microRNA (miR)-199a-5p in the regulati...

AIM To investigate the effect of Beclin 1, an autophagy gene, on the expression of angiopoietin (Ang) protein and the Tie-2 receptor in CaSki human cervical cancer cells. MATERIALS AND METHODS Beclin 1 overexpression (pcDNA3.1-Beclin1) and knockdown (pSUPER-Beclin1) plasmids were independently transfected into CaSki cells, and stably transfected cells were selected. Real-time fluorescence qua...