Loose bodies are fragments of cartilage or bone present in the synovial fluid. In the present study we assessed if loose bodies could be used as a source of autologous human chondrocytes for experimental purposes. Histochemical examination of loose bodies and differential enzymatic digestions were undertaken, the isolated cells were cultured in alginate bead microspheres and immunolocalisations...

Small-scale anaerobic digestion (AD) can be an effective organic waste management system that also provides energy for small businesses and rural communities. This study measured fuel production from digestions of single mixed feedstocks using unheated, 2 m3 digester operated continuously in a temperate climate over three years. Using local food waste, brewery grease agricultural residues, this...

Electron microscope examination of the adrenal cortex from three male human subjects revealed a special type of cell occurring in periendothelial spaces, in all adrenal cortex zones. It is a clear, spindle-shaped cell the principal cytoplasmic features of which are crystalline inclusions with a structure similar to that of the Reinke crystals of human testicular interstitial cells and an abunda...

This is the first report about flaxseed protein hydrolysates applied as media supplements in CHO cell culture. The were produced by three separate enzymatic digestions of proteins isolated from oil cake. enzymes used Alcalase, Neutrase, and Protamex, most efficient hydrolysis was achieved with Alcalase. tested a partial substitute for serum basal order to evaluate their effects on adherent IgG-...

The mechanism by which RapiGest SF improves the speed and completeness of digestion is illustrated in Figure 1. Mild protein denaturation opens protein structure and exposes the proteolytic sites to enzymatic cleavage. Enzymes are more resistant to denaturation than common proteins and remain active in RapiGest SF solutions. More complete denaturation of globular proteins can be accomplished by...

The amino acids of the chymotrypsi・n inhibitor (ECI) from the Ei:ythrina vm'iagata seeds have been sequeneed. The sequence was solyed by analysis of peptides derived from the pretein by enzymatic digestions with trypsin and &upbylocoeeus aureus V8 proteinase, as well as by chemical cleayage with o-iodosobenzoic acid. The ECI consists of 179 amine aeid residues with a pyroglutamic acid as the N-...

The amino acids of the chymotrypsi・n inhibitor (ECI) from the Ei:ythrina vm'iagata seeds have been sequeneed. The sequence was solyed by analysis of peptides derived from the pretein by enzymatic digestions with trypsin and &upbylocoeeus aureus V8 proteinase, as well as by chemical cleayage with o-iodosobenzoic acid. The ECI consists of 179 amine aeid residues with a pyroglutamic acid as the N-...

Amyloid-deposited light chain (AL) amyloidosis is correlated with the overproduction of a monoclonal immunoglobulin light chain protein by a B-lymphocyte clone. Since the amyloid fibril deposits in AL amyloidosis most often consist of the N-terminal fragments of the light chain, the majority of studies have focused on the determination of the primary structure of the protein, and reducing agent...

Microplastics (MPs) affect plankton (a basis of the trophic chain) and planktivorous fish can ingest them through food confusion or by transmission. Consensus to determine MPs in is lacking and, so, three digestion treatments were evaluated: Alkaline (potassium hydroxide) enzymatic (protease plus lipase) digestions, both combined with a hydrogen peroxide stage; an oxidative method using surfact...

background and objectives: urinary tract infection (uti) is one of the most common infections and a major cause of patient morbidity world wide. the gold standard for detection of bacterial pathogens in uti is culture of urine specimens. in order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in uti. ma...