Changes in the cell surface properties of amoebae of Dictyostelium discoideum during growth in different culture conditions have been studied by aqueous two-phase partitioning on a thin-layer countercurrent distribution apparatus. Changes in cell surface properties were not dependent on the source of nutrients but only on cell density. There was a progressive increase in cell surface hydrophobi...

Ž . Axenic rotifers Brachionus plicatilis Muller were cultured under aseptic conditions; they ̈ Ž . were fed either a bacteria-free artificial diet AD , or axenic Isochrysis galbana, or a combination of axenic Chlorella minutissima and the bacteria-free AD. The medium was inoculated with commercial bacterial additives or cultured strains of marine bacteria. The highest improvements in Ž . growth...

Effect of Drought, Flooding, and Potassium Stress on the Quantity and Composition of Root Exudates in Axenic Culture

We studied the resistance of Cenococcum geophilum and Suillus granulatus isolates to NaCl during growth under axenic culture conditions. C. geophilum isolates displayed variations in NaCl resistance; mycelial growth of most isolates was inhibited above 200mM. All isolates of S. granulatus were tolerant to high NaCl content.

Metagrowth is a new type of knowledge base developed to guide the experimental studies of culture conditions of obligate parasitic bacteria. We have gathered biological evidences giving possible clues to the development of the axenic (i.e. 'cell-free') growth of obligate parasites from various sources including published literature, genomic sequence information, metabolic databases and transpor...

Four species of Naegleria were tested for their ability to migrate under agarose. Pathogenic N. fowleri strains exhibited rapid locomotion at 37 degrees C. Environmental isolates of N. fowleri moved faster than clinical isolates which had been kept in axenic culture for longer periods, and this result was confirmed by using the 84-2205-7 strain kept in axenic culture for 1 or 5 months. Nonpatho...

The pine wilt nematode, Bursaphelenchus xylophilus, has been cultured axenically in vitro on soy peptone/yeast extract or modified Caenorhabditis medium supplemented with cholesterol and hemoglobin. Although growth, development and reproduction were best in soy peptone/yeast extract medium, satisfactory population size increases were observed in the chemically defined Caenorhaditis medium.

We report here the 3,586,065-bp draft genome of Geobacillus sp. LEMMY01, which was isolated (axenic culture) from a thermophilic chemolitoautotrophic consortium obtained from the site of a burning grass pile. The genome contains biosynthetic gene clusters coding for secondary metabolites, such as terpene and lantipeptide, confirming the biotechnological potential of this strain.

Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine.

The differences in fragility of microorganisms when treated in the French press are useful for destroying bacteria and mold contaminants, and assist in the preparation of axenic cultures of Chlorella.