apoptosis, a form of programmed cell death that occurs under physiological as well as pathological conditions, is characterized by morphological and biochemical features. while the importance of caspases in apoptosis is established, several noncaspase proteases (ca2+-dependent proteases) such as calpain may play a role in the execution of apoptosis. the calpain family consists of two major isof...

the aim of this study was to examine the effect of crude bile on the human hepg2 and ccrf-cem cell lines. cells were exposed to different dilutions of bile. antiproliferative effects were determined by the cytotoxic mtt assay. cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dutp nick end labeling (tunel) assay. bile administration resulted in dose-dependent c...

Objective(s): According to recent studies, valproate shows some protection against oxidative stress (OS) induced by neurotoxins. Current investigation tried to determine the possible ameliorating effects of sodium valproate (SV) against aluminum (Al)-induced cell death, apoptosis, mitochondrial membrane potential (MMP), and OS in PC12 cells.Materials ...

The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Bile administration resulted in dose-dependent c...

Objective(s):Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. T...

Quercetin compounds have antioxidant, anti-inflammatory and anticancer pharmacological functions. Long-term exposure to acrylamide (AA) can cause liver injury endanger human health. However, whether quercetin attenuate AA-induced the specific mechanism are not clear. Here, we studied structure-activity relationship of in reducing hepatotoxicity vivo vitro. In studies found that quercetin-like p...

Objective(s): The role of aluminum (Al) in the pathogenesis of neurodegenerative diseases has been implicated in several studies. However, the exact mechanisms of cytotoxic effects of Al have not been elucidated yet. The aim of this study was to investigate the effect of L-type calcium channel antagonist, nimodipine (NM), and lithium chloride (LiCl) on Al-induced toxicity in PC12 cells. Materia...

background and aims. there is no report on the apoptotic impact of allium sativum l. (garlic) on the oral squamous cell carcinoma (kb); hence, this study was designed to survey the apoptotic effects of garlic fresh juice (gfj) on the kb cells. materials and methods. mtt assay (microculture tetrazolium assay) was carried out to evaluate the cytotoxicity of gfj on kb cells. furthermore, tunel (te...

Spinal cord injury is pathologically characterized by the loss of motor function caused neurons apoptosis. Store-operated calcium entry (SOCE) widely known to dictate apoptosis various cell types. To examine SOCE in spinal and explore role apoptosis, patients with (SCI) SCI mouse models were included. Expression components apoptosis-related proteins examined Western blotting. Calcium imaging wa...

methods s. cerevisiae was cultured and then disrupted by sonication. after centrifugation, the harvested supernatant was considered to be a cytoplasmic extract. the protein concentration was determined by the biuret method and the extract was diluted to concentrations of 500, 1000, and 2000 µg protein/ml. the frequencies of apoptosis and necrosis were assessed in extract-treated k562 cells by e...