AIM To determine the usefulness of measuring amylase activity as an indicator of pancreatic disease in human immunodeficiency virus (HIV) positive patients. METHODS A prospective study of 129 ambulant HIV positive males. Total amylase, pancreatic amylase, and lipase activities were assayed using commercial test kits on an automated analyser. Samples with raised amylase were examined for the p...

Mycelial cell wall of Aspergillus oryzae M-13 grown in an alpha-amylase-forming medium could not bind alpha-amylase (Taka-amylase A, EC 3.2.1.1). However, by treatment with 1.0 n NaOH at 100 C for 30 min, the wall gained the ability to bind alpha-amylase. This phenomenon was caused by removal of a factor (designated as masking factor) which masked the binding site for alpha-amylase. The masking...

We evaluated three methods for serum amylase (EC 3.2.1.1) isoenzymes to determine whether they are interchangeable and to test their ability to discriminate between cystic fibrosis patients with and without pancreatic insufficiency. One method involved salivary amylase inhibitor (O), and two were polyacrylamide gel electrophoresis separations differing in method of detection--either direct zymo...

Human serous-type ovarian tumors contain an acidic isoenzyme of amylase. Previous attempts at purification of tumor amylases have yielded preparations contaminated with other proteins. The purification scheme presented here incorporates an affinity-chromatography procedure, with use of cycloheptaamylose linked to epoxy-activated Sepharose, that is specific for alpha-amylase (EC 3.2.1.1). Purifi...

BACKGROUND There have been many reports describing hyperamylasemia, with a salivary-type amylase phenotype, in patients with malignant tumors and/or multiple myelomas. In contrast, we have discovered and characterized a sialyl salivary-type amylase from multiple myeloma and/or lung cancer cells. This paper reports the first association of sialyl salivary-type amylase with ovarian cancer, discov...

Human saliva is an easily obtained, abundant secretion, but the native proteins in it seem to have been little studied. Only salivary amylase has been isolated in a pure state. In regard to electrophoresis, the phase boundary apparatus has been used by Blix' to study animal submaxillary mucin, by Bernfeld, Staub, and Fischer1 and Muus to test the homogeneity of purified salivary amylase, and by...

biochemical characteristics of α-amylase in the digestive system and head glands of cryptolaemus montrouzieri, a key predator of citrus mealybug, planococcus citri (pseudococcidae), were studied. the major isoform of α-amylase with the same molecular weight was detected in both gut and head glands loaded on polyacrylamide-starch gel electrophoresis. moreover, a minor band with much lower intens...

The novel a-amylase-pullulanase produced by Clostridium thermohydrosulfuricum E 101-69 was purified as two forms (I and II) from culture medium, by using gel filtration in 6 M-guanidine hydrochloride as the final step. Renatured a-amylase-pullulanase I and II had apparent Mr values of 370000+85000 and 330000+85000 respectively, as determined by native polyacrylamide-gradient-gel electrophoresis...

Organic constituents of human saliva vary around 0.5 g. per 100 ml. depending on the nature of the stimulus and the hour of collection. Most of the organic matter is protein, with mucin being the preponderant component. However, it is one of the smaller protein elements that has been the most widely studied, mainly because of its enzymatic action, that is, salivary amylase. Phase boundary elect...

Highlight: The level of pathogen alpha-amylase and plant beta-amylase activities could be components of plant-pathogen interaction associated with the resistance of triticale to Fusarium head blight. Triticale was used here as a model to recognize new components of molecular mechanism of resistance to Fusarium head blight (FHB) in cereals. Fusarium-damaged kernels (FDK) of two lines distinct in...