PURPOSE Recombinant adenoviral vectors are widely used in clinical and experimental studies to treat malignant tumors. Recently, host immune responses have been proposed as a major limitation in using adenoviral vectors for repeated gene delivery. We demonstrate another limitation unrelated to host immunity. EXPERIMENTAL DESIGN We repeatedly transduced an adenoviral vector expressing the huma...

NORPA-PLCb4 GENE EXPRESSION IN THE RAT BRAIN AND RETINA P. Roustsa*. M. Abitbol., F. Ribeaudeau*, C. M&nil, M. Gerard’, M. Vekemans”, J. Mall& and J.-L. Dufier*. WERTO, Faculle de medecim NeckeEafmteMnl&s, Univwsitl Ren6 Oesmtes,~i&m &age, 156 rue & Vaugimd, 75015 Paris. “Laboraoire de Oenetique Mokulaire d” D&elqpemat Hum&, Faculte d: dd&m ~eckm-Enfam-MaIdea UniversW Reti Descartes, 5ihe &age,...

After one year of pandemic SARS COV2, which started in December 2019, it is still considered a health disaster all nations. Despite vaccination efforts, the number infected people increased, also number COV2 variants increased. Certain vaccinations are approved under Emergency Use Authorization, including those dependent on mRNA technology, like developed by Pfizer-BioNTech and Moderna (FDA...

Gene therapy utilizing replication deficient adenoviral vectors represents a potentially promising approach to the treatment of brain tumors. Limited duration of systemic transgene expression and inefficient transduction following repeat systemic vector administration secondary to an effective anti-vector immune response limits the potential application of first generation adenoviral vectors. W...

Many approaches for treating hemophilia via gene transfer have been attempted in large animal models but all have potential drawbacks. Recombinant adenoviral vectors offer high-efficiency transfer of an episomal vector but have been plagued by the cytotoxicity/immunogenicity of early-generation vectors that contain viral genes. In our current study, we have used a nonintegrating helper-dependen...

OBJECTIVES The purpose of this study was to investigate whether a novel fibroblast growth factor-2 gene formulation, providing a localized and sustained availability of the adenoviral vector from a collagen-based matrix, in combination with CO 2 transmyocardial laser revascularization would lead to an enhanced angiogenic response and improved myocardial function. METHODS Fibroblast growth fac...

BACKGROUND AND PURPOSE Human brain arteriovenous malformation tissue displays increased levels of vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9, a tissue protease associated with various intracerebral hemorrhage (ICH). We hypothesized that increased MMP-9 was associated with ICH induced by vascular endothelial growth factor hyperstimulation and that this ...

The present study is devoted to the feasibility of expressing the single-domain mini-antibody (nanoantibody) selected from the library of sequences of the variable domains of special single-stranded antibodies derived from an immunized camel, a gene of which was introduced into eukaryotic cells within a recombinant adenoviral vector. A vector bearing the gene of a single-domain nanoantibody was...