Inflammation promotes endothelial dysfunction, but the underlying mechanisms remain poorly defined in vivo. Using translational vascular function testing myocardial infarction patients, a situation where inflammation is prevalent, and knock-out (KO) mouse models we demonstrate role for mitogen-activated-protein-kinases (MAPKs) dysfunction. Myocardial significantly lowers mitogen stress kinase 1...

Objective(s): Longan seeds have been used as a folk medicine in China. Longan seed extract (LSE) is known for antioxidative, antiproliferative, hypoglycemic, and hypouremic effects. However, its anti-inflammatory effect has not been shown. Materials and Methods: In this study, Sprague-Dawley (SD) rats were given LSE orally (vehicle, 10, and 30 mg/kg) for 3 days to its test anti-inflammatory eff...

Systemic sclerosis (SSc) is an idiopathic autoimmune disease with a heterogeneous clinical phenotype ranging from limited cutaneous involvement to rapidly progressive diffuse SSc. The most severe SSc and pathologic manifestations result uncontrolled fibrotic process involving the skin various internal organs. molecular mechanisms responsible for initiation progression of have not been fully elu...

The heterologous expression of human kinases in good purity and a monomeric, soluble active form can be challenging. Most the reported successful attempts are carried out insect cells as host. use E. coli for is limited to few usually facilitated by large solubility tags that limit biophysical studies affect protein–protein interactions. In this report, we evaluate methylotrophic yeast Pichia p...