A sensitive liquid-chromatography-electrospray tandem mass spectrometry multiclass method for determination of 45 veterinary compounds belonging to 9 different antibiotic groups, including aminoglicosides (4), β-lactams (13), diaminopyrimidines (1), fluoroquinolones (10), lincosamides (1), macrolides (5), pleuromutilins (1), sulfonamides (6) and tetracyclines (4), in water from breeding animal ...

The antimicrobial susceptibility profiles of Salmonella spp. isolated from livestock production systems in Sicily were determined. antibiotic sensitivity and broad-spectrum beta-lactamase strains assessed by detecting β-lactamases blaCTX-M IV, TEM, OXA SHV, blaCMY II, CTX-M I, DHA. In total, 93.3% showed multi-drug resistance (MDR). A total seven serotypes (i.e., Infantis, S. Typhimurium (monop...

We sought to determine the genetic and phenotypic antimicrobial resistance (AMR) profiles of commensal Klebsiella spp. circulating in Kenya by testing human stool isolates of 87 K. pneumoniae and three K. oxytoca collected at eight locations. Over one-third of the isolates were resistant to ≥3 categories of antimicrobials and were considered multidrug-resistant (MDR). We then compared the resis...

Pyrimethamine (Daraprim (Burroughs Wellcome) ; 2,4diamino-5-p-chlorophenyl-6-ethylpyrimidine) is used in the treatment and prevention of malaria (1). When used in combination with one of the sulfonamides, it is also effective in the treatment of toxoplasmosis (2) and coccidiosis (3). Pyrimethamine is one of a series of 2,4-diaminopyrimidines which have been shown to be antagonists of folic acid...

Recent studies have revealed that various derivatives of 2,4-diaminopyrimidine exhibit profound biochemical reactivity. For example, nearly all 2,4-diaminopyrimidines and their condensed ring systems inhibit the growth of LactobaciZZus casei with folic acid (1). 2,4-Diamino&p-chlorophenoxypyrimidine was found to be a potent antimalarial in that it was active against Plasmodium gallinaceum infec...

a series of novel 2-aminopyrimidine and 2-substituted-4,6-diaminopyrimidine derivatives have been synthesized and their anti platelet aggregation activities were assessed against adp and arachidonic acid- induced platelet aggregation in human plasma using light transmission aggregometry. none of the compounds showed satisfactory activity against the aggregation induced by adp. however, satisfac...

A simple multi-residue method was developed for detecting and quantifying 33 analytes from 13 classes of antibiotics (tetracyclines (3), quinolones (7), penicillins (3), ionophore coccidiostats (7), macrolides (3), sulfonamides (1), quinoxalines (2), phenicols (2), lincosamides (1), diaminopyrimidines (1), polypeptides (1), streptogramins (1) and pleuromutilins (1)) in animal feeds. Extraction ...

Drugs are commonly used in combinations larger than two for treating bacterial infection. However, it is generally impossible to infer directly from the effects of individual drugs the net effect of a multidrug combination. Here we develop a mechanism-independent method for predicting the microbial growth response to combinations of more than two drugs. Performing experiments in both Gram-negat...