Gejala kuning mirip seperti infeksi fitoplasma ditemukan pada pertanaman wortel di Jawa Barat. Penelitian bertujuan mengidentifikasi tanaman dan serangga wereng yang berasosiasi dengan gejala Bogor Bandung. Wereng terdiri atas 5 spesies daun (Hemiptera: Cicadellidae), yaitu Balclutha incisa (Matsumura), Orosius argentatus (Evans), Cicadulina bipunctata Empoascanara indica (Datta), Exitianus ind...

Phytoplasmas are dangerous bacteria severely infecting agricultural production worldwide. In the present study, identification of phytoplasmas tomato plants showing symptoms such as small leaves, flower abnormalities, stunting, witches' broom, and reddening was performed. Five plants, two symptomatic three asymptomatic, were tested to verify phytoplasma infection. Total DNA isolated from 5 leaf...

Phytoplasma phylogenetics has focused primarily on sequences of the non-coding 16S rRNA gene and the 16S-23S rRNA intergenic spacer region (16-23S ISR), and primers that enable amplification of these regions from all phytoplasmas by PCR are well established. In this study, primers based on the secA gene have been developed into a semi-nested PCR assay that results in a sequence of the expected ...