BACKGROUND Insulin/IGF-1 signaling plays a central role in longevity across phylogeny. In C. elegans, the forkhead box O (FOXO) transcription factor, DAF-16, is the primary target of insulin/IGF-1 signaling, and multiple isoforms of DAF-16 (a, b, and d/f) modulate lifespan, metabolism, dauer formation, and stress resistance. Thus far, across phylogeny modulation of mammalian FOXOs and DAF-16 ha...

FoxO transcription factors promote longevity across taxa. How they do so is poorly understood. In the nematode Caenorhabditis elegans, the A- and F-isoforms of the FoxO transcription factor DAF-16 extend life span in the context of reduced DAF-2 insulin-like growth factor receptor (IGFR) signaling. To elucidate the mechanistic basis for DAF-16/FoxO-dependent life span extension, we performed an...

Bromination of visnagin (1) afforded 9-bromovisnagin (2) which on its alkaline hydrolysis afforded the 3-acetyl benzofuran derivative (3). The condensation of (3) with hydrazine hydrate, phenylhydrazine and/or hydroxylamine hydrochloride afforded the corresponding pyrazole derivatives (4a, b) and isoxazole derivative (4c). On the other hand, when compound 3 was condensed with some aromatic alde...

The novel 1-(2-fluorovinyl)-4-quinolone-3-carboxylic acid derivatives Z-15a-c, E-15a-c, Z-16a-c, and E-16a-c, conformationally restricted analogues of fleroxacin (5), were synthesized, and their in vitro antibacterial activity was evaluated. A dehydrosulfenylation of a 2-fluoro-2-[(4-methoxyphenyl)sulfinyl]ethyl group was employed as a key step for the construction of a 2-fluorovinyl group at t...

Forty nine adult male Sprague Dawley rats were selected for rapid learning in a T-maze to study the effects of BR-16A, a herbal preparation on ECS-induced retrograde amnesia. Half the rats received 200 mg/kg BR-16A and the other half received vehicle alone. The rats were trained to run into the enclosed chamber of either the left or the right arm on a T-maze; a food pellet served as a reward in...

AUTHORS Julien ESTAGER / CERTECH ASBL, RUE JULES BORDET ZONE INDUSTRIELLE C, SENEFFE Jessica PLECK / CERTECH ASBL, RUE JULES BORDET ZONE INDUSTRIELLE C, SENEFFE Jean-Christophe MONBALIU / CENTER FOR INTEGRATED TECHNOLOGY AND ORGANIC SYNTHESIS CITOS UNIVERSITY OF LIEGE, BUILDING B6A, ROOM 3/16A ALLEE DU SIX AOUT 13, LIEGE (SART TILMAN) Romaric GERARDY / CENTER FOR INTEGRATED TECHNOLOGY AND ORGAN...

Parkinson's disease (PD) is a neurodegenerative disease characterized by the selective loss of dopamine (DA) neurons of the substantia nigra pars compacta (SNc). The events, which trigger and/or mediate the loss of nigral DA neurons, however, remain unclear. Neuroleptic-induced catalepsy has long been used as an animal model for screening drugs for Parkinsonism. Administration of haloperidol (1...

The purpose of this study was to determine whether the BACTEC NR-16A and NR-17A media were more effective than the BACTEC NR-6A and NR-7A media in recovering organisms from the blood of patients undergoing antimicrobial therapy. A total of 986 sets of four blood culture bottles were compared, giving 141, 174, 93, and 104 isolates with BACTEC NR-6A, NR-16A, NR-7A, and NR-17A, respectively. BACTE...

To further study the steroid binding site of 20&hydroxysteroid dehydrogenase (EC 1.1.1.53) by affinity labeling, 21[2’JH]bromoacetylaminoprogesterone was synthesized. The steroid inactivates the enzyme in a time-dependent and irreversible manner which follows pseudo-first order kinetics. Incorporation of 2 mol of 21-[2’-3Hlbromoacetylaminoprogesterone accompanies inactivation of 1 mol of enzyme...

1,3,5(10),16-Estratetraen-3-01 (estratetraenol) that has previotisly been found in the urine of women in late pregnancy and proved to be biosynthesized in human placenta from 4,16-androstadien-3-one, a hepatic metabolite of testosterone, was converted under aerobic conditions by female rat liver microsomes into estratetraenol 16a,l7a-epoxide, estratetraenol 16P,17P-epoxide, 16,17-epiestriol, an...