Highly purified preparations of Hageman factor, a potent clotpromoting agent in normal mammalian plasma, had a sedimentation coefficient of approximately 5S before activation. After activation, the Hageman factor behaved as a much less soluble or larger molecule during ultracentrifugation and gel filtration. No significant change in sedimentation behavior was noted when the Hageman factor in pl...

Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose...

The sedimentation of hard spheres in a Newtonian solvent is studied as a function of Péclet number in the low-concentration limit. Two functional forms for the sedimentation velocity as a function of particle concentration are realized in the limit of high and low Péclet numbers. We argue that a more ordered phase occurs for large Péclet numbers. Measurements of settling in sheared suspensions ...