Double stranded human globin cDNA was synthesized by use of viral reverse transcriptase from globin mRNA of cord blood of premature infants requiring exchange transfusions. The cDNA was introduced into plasmids and the recombinant DNA plasmids used to transform E. coli X1776. A number of transformants were obtained. Plasmid DNA from selected colonies was isolated and characterized for the type ...

Jaundice is the most common medical problem affecting babies in the first week of life. Phototherapy was introduced as a treatment for jaundice in 1958, and its widespread use has reduced the need for exchange transfusion. Bilirubin charts or mathematical formulae based on gestation may be used to guide treatment decisions for hyperbilirubinaemia. We were interested in current practices and rec...

Kemball, M. L., McIver, C., Milner, R. D. G., Nourse, C. H., Schiff, D., Tiernan, J. R. (1970). Archives of Disease in Childhood, 45, 696. Neonatal hypoglycaemia in infants of diabetic mothers given sulphonylurea drugs in pregnancy. Three infants whose diabetic mothers were given chlorpropamide and one infant whose diabetic mother was given acetohexamide up to the time of delivery were studied ...

Influence of altered blood oxygen affinity on maximal performance ability was evaluated in trained rats exercising to exhaustion in a graded treadmill test. Modification of blood oxygen affinity was achieved both by 2,3-diphosphoglycerate depletion, accomplished by exposure of animals to CO(2) and by exchange transfusion with blood exposed to bisulfite or stored in acid citrate dextrose, and by...

cepted reference method for total bilirubin determination is currently available. Thus, the widely used DPD procedure was chosen as the comparison method in this study. Quality specifications in terms of precision and accuracy met CLIA (11 ) as well as European regulatory requirements (12 ). In the patient samples, the measured bilirubin values covered the physiologically relevant range (85–400...

OBJECTIVE To describe pregnancy outcomes for pregnant women with sickle cell disease (SCD) receiving prophylactic transfusions. STUDY DESIGN This retrospective case-control study compared pregnancy outcomes among women with SCD receiving prophylactic transfusions and women without any hemoglobinopathy, matched for ethnicity, parity, age and hospital. RESULTS The study included two groups of...

The objective of this study was to report on a breakthrough of Plasmodium falciparum infection following a military exercise in central Kenya and the treatment regimens used. A series of case reports are presented from the three UK hospitals involved. Among 150 British soldiers who had been on exercises for five weeks in central Kenya, taking proguanil/chloroquine anti-malarial prophylaxis, sev...

rFHE LEUKEMIAS OF MAX are commonly considered as diseases characterized by \videsprea(l proliferation of leukocytes and their precursors in the tissues ol the body) This concept has greatly influenced thought and investigations concerning this group of diseases, although indisputable proof of hyperproduction of the white 1)100(1 cells in leukemia is not recorded in the literature. More recently...

Although macroglobulinemia of Waldenstr6m is a rare disorder, the physicochemical modification of macroglobulins therein has broad implications, since similar large proteins may be involved in the pathogenesis of symptoms associated with a variety of relatively common diseases; i.e., Rh isosensitization, hemolysis due to cold agglutinins, and so on. Also, a number of normally occurring proteins...

It is now clearly established that the transfusion of blood from variant CJD (v-CJD) infected individuals can transmit the disease. Since the number of asymptomatic infected donors remains unresolved, inter-individual v-CJD transmission through blood and blood derived products is a major public health concern. Current risk assessments for transmission of v-CJD by blood and blood derived product...