Concentration of 5 gamma g(ml or less of 5-Auorodeoxyuridine (FDC) and of 5-bromodeoxyridine (BDU) inhibited the growth of adenovirus type 5 and of vaccinia virus in ERK and chick ell1bryo cells, provided that adequate time was allowed for the inhibitors to act. With the same proviso, FD {T and BDU also inhibited the growth of ERK cells. Cnder similar conditions of cultivation, concentrations o...

Numerous mammalian proto-oncogene and other growth-regulatory transcripts are upregulated in malignancy due to abnormal mRNA stabilization. In hepatoma cells expressing a hepatitis C virus (HCV) subgenomic replicon, we found that the viral nonstructural protein 5A (NS5A), a protein known to bind to viral RNA, also bound specifically to human cellular transcripts that encode regulators of cell g...

Mutations of the conserved residues of influenza virus neuraminidase (NA) that are associated with NA inhibitor (NAI) resistance decrease the sialidase activity and/or stability of the NA, thus compromising viral fitness. In fact, clinically derived NAI-resistant variants with different NA mutations have shown different transmissibilities in ferrets (M. L. Herlocher, R. Truscon, S. Elias, H. Ye...

We have previously described isolation and preliminary identification of a virus related to Dugbe virus (DUGV), family Bunyaviridae, genus Nairovirus. Six isolates of the virus were obtained from pools of Amblyomma gemma and Rhipicephalus pulchellus ticks collected from hides of cattle in Nairobi, Kenya, in October 1999. We report results of further characterization of this virus, including gro...

We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P(-)) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was r...