'Universal' primers have been used to amplify the nontranscribed spacer (NTS) of the ribosomal 5S gene of avocado genotypes by the polymerase chain reaction (PCR). A rich size heterogeneity of NTSs of individual avocado genotypes was detected after high resolution polyacrylamide gel electrophoresis and silver staining. Specific sizes of NTS seem to be common for members of each horticultural ra...

We described a method for PCR amplification of unknown flanking genomic DNA fragments. This method is a combination of PCR with "endtr imming method" and "cassettes and cassette-primers method". In this method, genomic DNA was digested with three different groups of restriction enzymes. DNA in group 1 was digested with BamHI, Bglll, Fbal, or Mbol. DNA in group 2 was digested with Blnl, Nhel, Sp...

Aquatic environmental DNA (eDNA) studies have considerably taken off during the last 10 years, promptly establishing eDNA as a new approach to monitoring ecological status of European surface waters. On one hand due numerous biological methods based on aquatic species, other development group-specific primers that allowed discrimination between species or at least genera. Understandably, rapid ...

this study utilized molecular methods to identify 22 isolates from traditional dairy products of azerbaijan. pcr was carried out using the universal primers of 16s rdna and a fragment of ~1500bp was amplified. amplified ribosomal dna restriction analysis was performed using bsuri, mspi, hindiii, taqi and bsp143i. ardra results were compared to the restriction profile in enzymatic restriction an...

Data from European studies indicate that in humans, particular Borrelia burgdorferi genospecies may be associated with specific clinical manifestations of Lyme disease. Infections by B. burgdorferi sensu stricto tend to lead to arthritic symptoms, whereas infections by B. garinii appear to cause neurologic complications. Late cutaneous manifestations (acrodermatitis) appear to be associated wit...

Ten strains of Aeromonas hydrophila isolated from water samples were characterized by various methods including biochemical and physiological tests, as well as PCR-RFLP analysis with Universal 16s rRNA primers. Biochemical characterization and PCR-RFLP analysis proved to be useful, rapid and reliable method for identifying widely distributed Aeromonas spp. from environmental samples. The signif...

self etching primers, due to ease of manipulation, have been extensively used in recent years. these self etching primers containing a relatively weak acid, may not provide an optimal bond strength. the purpose of the present in-vitro investigation was to evaluate the effects of 37% phosphoric acid application prior to applying self etching primers on composite bond strength. in this experiment...

A phylogenetic study of the Opopanax and Crenosciadium was conducted to elucidate their circumscription infrageneric relationships. Sequence data from nrDNA ITS, cpDNA matK trnL-F were used determine relationship. Polymerase Chain Reaction (PCR) amplifications all regions performed using universal primers. Maximum Likelihood (ML) trees constructed PAUP* software, another tree ITS sequences show...